Experimental applications of in situ liver perfusion machinery for the study of liver disease

Won Mook Choi, Hyuk Soo Eun, Young-Sun Lee, Sun Jun Kim, Myung Ho Kim, Jun Hee Lee, Young Ri Shim, Hee Hoon Kim, Ye Eun Kim, Hyon Seung Yi, Won Il Jeong

Research output: Contribution to journalArticle

Abstract

The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahe-patic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

Original languageEnglish
Pages (from-to)45-55
Number of pages11
JournalMolecules and cells
Volume42
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Liver Diseases
Perfusion
Liver
Inferior Vena Cava
Hepatocytes
Density Gradient Centrifugation
Poisons
Portal Vein
Constriction
Catheterization
Cell Movement
Vertebrates
Immune System
Cell Survival
Hemodynamics
Proteins

Keywords

  • Hemodynamics
  • Immune cell
  • In situ perfusion
  • Liver disease
  • Metabolism

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Experimental applications of in situ liver perfusion machinery for the study of liver disease. / Choi, Won Mook; Eun, Hyuk Soo; Lee, Young-Sun; Kim, Sun Jun; Kim, Myung Ho; Lee, Jun Hee; Shim, Young Ri; Kim, Hee Hoon; Kim, Ye Eun; Yi, Hyon Seung; Jeong, Won Il.

In: Molecules and cells, Vol. 42, No. 1, 01.01.2019, p. 45-55.

Research output: Contribution to journalArticle

Choi, WM, Eun, HS, Lee, Y-S, Kim, SJ, Kim, MH, Lee, JH, Shim, YR, Kim, HH, Kim, YE, Yi, HS & Jeong, WI 2019, 'Experimental applications of in situ liver perfusion machinery for the study of liver disease', Molecules and cells, vol. 42, no. 1, pp. 45-55. https://doi.org/10.14348/molcells.2018.0330
Choi, Won Mook ; Eun, Hyuk Soo ; Lee, Young-Sun ; Kim, Sun Jun ; Kim, Myung Ho ; Lee, Jun Hee ; Shim, Young Ri ; Kim, Hee Hoon ; Kim, Ye Eun ; Yi, Hyon Seung ; Jeong, Won Il. / Experimental applications of in situ liver perfusion machinery for the study of liver disease. In: Molecules and cells. 2019 ; Vol. 42, No. 1. pp. 45-55.
@article{45cb94bec3694e84a378c7518403aa2b,
title = "Experimental applications of in situ liver perfusion machinery for the study of liver disease",
abstract = "The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahe-patic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.",
keywords = "Hemodynamics, Immune cell, In situ perfusion, Liver disease, Metabolism",
author = "Choi, {Won Mook} and Eun, {Hyuk Soo} and Young-Sun Lee and Kim, {Sun Jun} and Kim, {Myung Ho} and Lee, {Jun Hee} and Shim, {Young Ri} and Kim, {Hee Hoon} and Kim, {Ye Eun} and Yi, {Hyon Seung} and Jeong, {Won Il}",
year = "2019",
month = "1",
day = "1",
doi = "10.14348/molcells.2018.0330",
language = "English",
volume = "42",
pages = "45--55",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

TY - JOUR

T1 - Experimental applications of in situ liver perfusion machinery for the study of liver disease

AU - Choi, Won Mook

AU - Eun, Hyuk Soo

AU - Lee, Young-Sun

AU - Kim, Sun Jun

AU - Kim, Myung Ho

AU - Lee, Jun Hee

AU - Shim, Young Ri

AU - Kim, Hee Hoon

AU - Kim, Ye Eun

AU - Yi, Hyon Seung

AU - Jeong, Won Il

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahe-patic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

AB - The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahe-patic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

KW - Hemodynamics

KW - Immune cell

KW - In situ perfusion

KW - Liver disease

KW - Metabolism

UR - http://www.scopus.com/inward/record.url?scp=85060934629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060934629&partnerID=8YFLogxK

U2 - 10.14348/molcells.2018.0330

DO - 10.14348/molcells.2018.0330

M3 - Article

VL - 42

SP - 45

EP - 55

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -