Exposure to etoxazole induces mitochondria-mediated apoptosis in porcine trophectoderm and uterine luminal epithelial cells

Wonhyoung Park, Whasun Lim, Sunwoo Park, Kwang Youn Whang, Gwonhwa Song

Research output: Contribution to journalArticle

Abstract

Etoxazole is an organofluorine insecticide widely used in agriculture. Exposure to insecticides is a serious environmental problem owing to their cytotoxic effects in humans and animals. Reproductive toxicity of various organofluorine insecticides have been shown in previous studies. However, few studies have evaluated the toxicity of etoxazole in mammals. We aimed to examine the toxic effects of etoxazole in porcine trophectoderm (pTr) and uterine luminal epithelial (pLE) cells. To estimate the effects of etoxazole, we conducted assays after treatment with multiple concentration of etoxazole (0, 2, 4, 6 and 9 μM) to pTr and pLE cells for 0–72 h. Etoxazole decreased the cell proliferation, viability, and migration of pTr and pLE cells. Further, etoxazole induced apoptosis via cell cycle arrest and disruption of mitochondrial membrane potential. We also found that pro-apoptotic proteins and endoplasmic reticulum (ER) stress-response proteins were activated in response to etoxazole. Finally, we observed that etoxazole altered the PI3K/AKT and MAPK signaling pathways and the mRNA expression of genes associated with implantation. Collectively, these results suggest that etoxazole disrupts normal cellular physiology and might cause early implantation failure. Etoxazole might cause implantation failure via growth retardation and induction of cell death in porcine trophectoderm and endometrial luminal epithelium.

Original languageEnglish
Article number113480
JournalEnvironmental Pollution
Volume257
DOIs
Publication statusPublished - 2020 Feb

Fingerprint

Insecticides
Mitochondria
Cell death
Swine
Epithelial Cells
Apoptosis
Toxicity
Proteins
Mammals
Physiology
Cell proliferation
Agriculture
Assays
Animals
Genes
Cells
Membranes
etoxazole
Apoptosis Regulatory Proteins
Endoplasmic Reticulum Stress

Keywords

  • Apoptosis
  • Endometrium
  • Etoxazole
  • Pregnancy
  • Trophectoderm

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Exposure to etoxazole induces mitochondria-mediated apoptosis in porcine trophectoderm and uterine luminal epithelial cells. / Park, Wonhyoung; Lim, Whasun; Park, Sunwoo; Whang, Kwang Youn; Song, Gwonhwa.

In: Environmental Pollution, Vol. 257, 113480, 02.2020.

Research output: Contribution to journalArticle

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