Expressed sequence tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]

C. S. Jang, J. Y. Kim, J. W. Haam, M. S. Lee, D. S. Kim, Y. W. Li, Yong Weon Seo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Of the 16 known biotypes of the Hessian fly [Mayetiola destructor (Say)], biotype L is recognized as being the most virulent. We have previously reported the development of near-isogenic lines (NILs) (BC3F3:4) by backcross introgression (Coker797*4/Hamlet) that differed by the presence or absence of the H21 gene on 2RL chromatin. Florescence in situ hybridization analysis revealed introgressed 2RLs in NILs possessing the H21 gene, but no signal was detected in NILs lacking 2RL. As part of an approach to elucidate molecular interactions between plants and the Hessian fly, a cDNA library from NILs with H21 infested by larvae of biotype L of the Hessian fly was constructed for expressed sequence tag (EST) analysis. Of 1,056 sequenced reactions attempted, 919 ESTs produced some lengths of readable sequences. Based on their putative identification, 730 ESTs that showed significant similarity with amino acid sequences registered in the gene bank were divided into 13 functional categories. Defense- and stress-related genes represented about 16.1%, including protease inhibition, oxidative burst, lignin synthesis, and phenylpropanoid metabolism. EST clones obtained from the cDNA library may provide a clue to the molecular interactions between plant and larva of the Hessian fly larval infestation.

Original languageEnglish
Pages (from-to)150-158
Number of pages9
JournalPlant Cell Reports
Volume22
Issue number2
DOIs
Publication statusPublished - 2003 Sep 1

Fingerprint

Mayetiola destructor
translocation lines
expressed sequence tags
rye
isogenic lines
wheat
biotypes
larvae
cDNA libraries
gene banks
genes
phenylpropanoids
introgression
in situ hybridization
chromatin
lignin
proteinases
amino acid sequences
clones
metabolism

Keywords

  • 2RL
  • EST
  • H21
  • Hessian fly
  • Wheat-rye translocation

ASJC Scopus subject areas

  • Plant Science

Cite this

Expressed sequence tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]. / Jang, C. S.; Kim, J. Y.; Haam, J. W.; Lee, M. S.; Kim, D. S.; Li, Y. W.; Seo, Yong Weon.

In: Plant Cell Reports, Vol. 22, No. 2, 01.09.2003, p. 150-158.

Research output: Contribution to journalArticle

Jang, C. S. ; Kim, J. Y. ; Haam, J. W. ; Lee, M. S. ; Kim, D. S. ; Li, Y. W. ; Seo, Yong Weon. / Expressed sequence tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]. In: Plant Cell Reports. 2003 ; Vol. 22, No. 2. pp. 150-158.
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