Expressed sequence tags from wheat roots under hypoxia

T. G. Lee, C. S. Jang, J. Y. Kim, R. C. Seong, I. G. Kim, D. S. Kim, Yong Weon Seo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hypoxia due to waterlogging adversely affects agricultural crops in many parts of the world and severely impacts overall annual production levels. To evaluate the response of wheat roots to a controlled hypoxic environment at the level of transcription, a cDNA library was constructed using hypoxia-stressed wheat. A total of 1274 clones (94.8%) out of 1344 sequences were found to be at least 50 bp long. Phrap assembly using high quality sequences formed 879 sets of non-overlapping sequences. Based on BLASTx analysis against the nonredundant database, a total of 494 unigenes showed a high homology to the registered amino acid sequences of plant (E < e-10). A total of 112 unigenes, which were not matched to any wheat ESTs in the database, were detected. A functional classification of 118 clones whose transcript levels were evaluated under hypoxia was performed according to gene ontology. These results may provide useful information for further investigations to better understand the response of wheat roots to waterlogging.

Original languageEnglish
Pages (from-to)659-668
Number of pages10
JournalRussian Journal of Plant Physiology
Volume54
Issue number5
DOIs
Publication statusPublished - 2007 Sep 1

Fingerprint

expressed sequence tags
hypoxia
unigenes
wheat
flooded conditions
clones
cDNA libraries
amino acid sequences
transcription (genetics)
taxonomy
crops
genes

Keywords

  • Expressed sequence tags
  • Hypoxia
  • Roots
  • Triticum aestivum

ASJC Scopus subject areas

  • Plant Science

Cite this

Lee, T. G., Jang, C. S., Kim, J. Y., Seong, R. C., Kim, I. G., Kim, D. S., & Seo, Y. W. (2007). Expressed sequence tags from wheat roots under hypoxia. Russian Journal of Plant Physiology, 54(5), 659-668. https://doi.org/10.1134/S1021443707050147

Expressed sequence tags from wheat roots under hypoxia. / Lee, T. G.; Jang, C. S.; Kim, J. Y.; Seong, R. C.; Kim, I. G.; Kim, D. S.; Seo, Yong Weon.

In: Russian Journal of Plant Physiology, Vol. 54, No. 5, 01.09.2007, p. 659-668.

Research output: Contribution to journalArticle

Lee, TG, Jang, CS, Kim, JY, Seong, RC, Kim, IG, Kim, DS & Seo, YW 2007, 'Expressed sequence tags from wheat roots under hypoxia', Russian Journal of Plant Physiology, vol. 54, no. 5, pp. 659-668. https://doi.org/10.1134/S1021443707050147
Lee TG, Jang CS, Kim JY, Seong RC, Kim IG, Kim DS et al. Expressed sequence tags from wheat roots under hypoxia. Russian Journal of Plant Physiology. 2007 Sep 1;54(5):659-668. https://doi.org/10.1134/S1021443707050147
Lee, T. G. ; Jang, C. S. ; Kim, J. Y. ; Seong, R. C. ; Kim, I. G. ; Kim, D. S. ; Seo, Yong Weon. / Expressed sequence tags from wheat roots under hypoxia. In: Russian Journal of Plant Physiology. 2007 ; Vol. 54, No. 5. pp. 659-668.
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