Expression and changes of calbindin D-28k immunoreactivity in the ventral horn after transient spinal cord ischemia in rabbits

Jae Chul Lee, In Koo Hwang, Jun Hwi Cho, Seung Myung Moon, Tae Cheon Kang, Won Ki Kim, Moo Ho Won

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We examined ischemia-related changes of calbindin D-28k (CB) immunoreactivity in L 7 of the spinal ventral horn after transient spinal cord ischemia in rabbits. In the sham-operated group, CB immunoreactivity was not present in the spinal ventral horn, but CB immunoreactivity was detectable in the dorsal horn. CB immunoreactivity was detectable in the ventral horn at 30 min after ischemia: the CB immunoreactivity was found in glial cells identified as astrocytes. At 1 h after ischemia, CB immunoreactivity was highest and present at a few somata located in the lamina VII as well as many glial cells. CB immunoreactivity was lower in the lamina VII at 3 h after ischemia compared to 1 h post-ischemic group. By 2 days after ischemia, CB immunoreactivity was decreased in this region. In addition, the result of Western blot result showed the pattern of CB expression similar to that of immunohistochemistry. In conclusion, the ischemia-related changes of CB immunoreactivity in neurons and glial cells in the ischemic spinal ventral horn in rabbits may be related to modulation of intracellular calcium following transient ischemia.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalNeuroscience Letters
Volume369
Issue number2
DOIs
Publication statusPublished - 2004 Oct 14
Externally publishedYes

Keywords

  • Astrocytes
  • Calbindin D-28k
  • Calcium-binding proteins
  • Excitotoxicity
  • Interneurons
  • Rabbit
  • Spinal cord ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

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