Expression and deletion analyses of cspE encoding cold-shock protein E in Acinetobacter oleivorans DR1

Jisun Kim, Sunhee Ha, Woojun Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were functionally important during cold shock because of their gradual upregulation during a temperature decrease under our assay conditions. cspE also showed higher expression during alkane degradation and antibiotic exposure. The transcriptional start site of the cspE gene was determined using 5′ rapid amplification of complementary DNA ends. Next, promoter analysis using numerous constructed gfp reporter strains containing deleted fragments of cspE upstream regions identified possible 5′ untranslated region (UTR) cis-DNA elements that could be involved in modulating cspE expression. Deletion of cspE led to a growth defect and enhanced biofilm formation, but only at a low temperature. Collectively, our findings show the importance of CspE during cold shock, dynamic regulation of cspE expression under various stressful conditions and a possible 5′-UTR cis-DNA element for regulation of cspE expression. These data provide molecular insight into cspE gene expression during cold-shock adaptation in soil bacteria.

Original languageEnglish
JournalResearch in Microbiology
DOIs
Publication statusAccepted/In press - 2018 Jan 1

Fingerprint

Cold Shock Proteins and Peptides
Acinetobacter
Shock
5' Untranslated Regions
Genes
Temperature
Alkanes
DNA
Biofilms
Up-Regulation
Soil
Complementary DNA
Genome
Anti-Bacterial Agents
Bacteria
Gene Expression
Amino Acids
Growth

Keywords

  • Acinetobacter
  • Cold-shock protein
  • CspA
  • CspE
  • Soil bacteria
  • Transcription

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Expression and deletion analyses of cspE encoding cold-shock protein E in Acinetobacter oleivorans DR1. / Kim, Jisun; Ha, Sunhee; Park, Woojun.

In: Research in Microbiology, 01.01.2018.

Research output: Contribution to journalArticle

@article{6ede04cd4ae1491eb36ff7467715617b,
title = "Expression and deletion analyses of cspE encoding cold-shock protein E in Acinetobacter oleivorans DR1",
abstract = "Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were functionally important during cold shock because of their gradual upregulation during a temperature decrease under our assay conditions. cspE also showed higher expression during alkane degradation and antibiotic exposure. The transcriptional start site of the cspE gene was determined using 5′ rapid amplification of complementary DNA ends. Next, promoter analysis using numerous constructed gfp reporter strains containing deleted fragments of cspE upstream regions identified possible 5′ untranslated region (UTR) cis-DNA elements that could be involved in modulating cspE expression. Deletion of cspE led to a growth defect and enhanced biofilm formation, but only at a low temperature. Collectively, our findings show the importance of CspE during cold shock, dynamic regulation of cspE expression under various stressful conditions and a possible 5′-UTR cis-DNA element for regulation of cspE expression. These data provide molecular insight into cspE gene expression during cold-shock adaptation in soil bacteria.",
keywords = "Acinetobacter, Cold-shock protein, CspA, CspE, Soil bacteria, Transcription",
author = "Jisun Kim and Sunhee Ha and Woojun Park",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.resmic.2018.04.011",
language = "English",
journal = "Research in Microbiology",
issn = "0923-2508",
publisher = "Elsevier Masson SAS",

}

TY - JOUR

T1 - Expression and deletion analyses of cspE encoding cold-shock protein E in Acinetobacter oleivorans DR1

AU - Kim, Jisun

AU - Ha, Sunhee

AU - Park, Woojun

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were functionally important during cold shock because of their gradual upregulation during a temperature decrease under our assay conditions. cspE also showed higher expression during alkane degradation and antibiotic exposure. The transcriptional start site of the cspE gene was determined using 5′ rapid amplification of complementary DNA ends. Next, promoter analysis using numerous constructed gfp reporter strains containing deleted fragments of cspE upstream regions identified possible 5′ untranslated region (UTR) cis-DNA elements that could be involved in modulating cspE expression. Deletion of cspE led to a growth defect and enhanced biofilm formation, but only at a low temperature. Collectively, our findings show the importance of CspE during cold shock, dynamic regulation of cspE expression under various stressful conditions and a possible 5′-UTR cis-DNA element for regulation of cspE expression. These data provide molecular insight into cspE gene expression during cold-shock adaptation in soil bacteria.

AB - Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were functionally important during cold shock because of their gradual upregulation during a temperature decrease under our assay conditions. cspE also showed higher expression during alkane degradation and antibiotic exposure. The transcriptional start site of the cspE gene was determined using 5′ rapid amplification of complementary DNA ends. Next, promoter analysis using numerous constructed gfp reporter strains containing deleted fragments of cspE upstream regions identified possible 5′ untranslated region (UTR) cis-DNA elements that could be involved in modulating cspE expression. Deletion of cspE led to a growth defect and enhanced biofilm formation, but only at a low temperature. Collectively, our findings show the importance of CspE during cold shock, dynamic regulation of cspE expression under various stressful conditions and a possible 5′-UTR cis-DNA element for regulation of cspE expression. These data provide molecular insight into cspE gene expression during cold-shock adaptation in soil bacteria.

KW - Acinetobacter

KW - Cold-shock protein

KW - CspA

KW - CspE

KW - Soil bacteria

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=85047177236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047177236&partnerID=8YFLogxK

U2 - 10.1016/j.resmic.2018.04.011

DO - 10.1016/j.resmic.2018.04.011

M3 - Article

C2 - 29751060

AN - SCOPUS:85047177236

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

ER -