Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps

Sang Hag Lee, Ji Hoon Park, Hak Hyun Jung, Seung Hoon Lee, Joon Whan Oh, Heung Man Lee, Hyun Soo Jun, Woo Jin Cho, Jae Yong Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Conclusions. Our results indicate that the electrogenic kidney Na +/HCO 3 cotransporter (kNBC), KCl cotransporter (KCC1 and -4) and Ca 2+-activated Cl - channel (CaCC1, -2, -3) mRNAs are expressed in normal nasal mucosa and nasal polyp, suggesting that altered expression of all CaCC mRNAs in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells. Objective. Electrolyte transport by nasal epithelia has been suggested to be important for controlling the quantity and composition of the nasal fluid and may play an important role in the development of nasal polyps. Transepithelial transport of ions and water in various fluid-transporting epithelia is strictly dependent on the localization of specific membrane proteins in the polarized epithelial cells. In this study we investigated the expression and distribution of mRNA transcripts for kNBC, pancreatic NBC, KCC1, -2, -3, -4 and CaCC1, -2, -3 gene families in human nasal mucosa and nasal polyp. Material and methods. The expression and localization of these gene families were investigated in inferior turbinate tissues and nasal polyp using reverse transcriptase polymerase chain reaction (RT-PCR), semiquantitative RT-PCR and in situ hybridization. Results. mRNAs for kNBC, KCC1 and -4 and all the CaCC families (CaCC1, -2 and -3) are expressed in human turbinate mucosa and nasal polyp. The expression levels of kNBC and KCC1 and -4 mRNAs did not differ between nasal mucosa and nasal polyp. However, the expression levels of all the CaCC genes were significantly decreased in nasal polyp. In situ hybridization revealed that the expression of these genes was mainly localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyp.

Original languageEnglish
Pages (from-to)745-752
Number of pages8
JournalActa Oto-Laryngologica
Volume125
Issue number7
DOIs
Publication statusPublished - 2005 Aug 5

Fingerprint

Nasal Polyps
Nasal Mucosa
Ion Transport
Messenger RNA
Turbinates
Reverse Transcriptase Polymerase Chain Reaction
Electrolytes
In Situ Hybridization
Mucous Membrane
Epithelial Cells
Gene Expression
Water
Nose
Genes
Membrane Proteins
Epithelium
Kidney

Keywords

  • Ca -activated channel
  • In situ hybridization
  • KCl cotransporter
  • Na /HCO cotransporter
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps. / Lee, Sang Hag; Park, Ji Hoon; Jung, Hak Hyun; Lee, Seung Hoon; Oh, Joon Whan; Lee, Heung Man; Jun, Hyun Soo; Cho, Woo Jin; Lee, Jae Yong.

In: Acta Oto-Laryngologica, Vol. 125, No. 7, 05.08.2005, p. 745-752.

Research output: Contribution to journalArticle

Lee, Sang Hag ; Park, Ji Hoon ; Jung, Hak Hyun ; Lee, Seung Hoon ; Oh, Joon Whan ; Lee, Heung Man ; Jun, Hyun Soo ; Cho, Woo Jin ; Lee, Jae Yong. / Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps. In: Acta Oto-Laryngologica. 2005 ; Vol. 125, No. 7. pp. 745-752.
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T1 - Expression and distribution of ion transport mRNAs in human nasal mucosa and nasal polyps

AU - Lee, Sang Hag

AU - Park, Ji Hoon

AU - Jung, Hak Hyun

AU - Lee, Seung Hoon

AU - Oh, Joon Whan

AU - Lee, Heung Man

AU - Jun, Hyun Soo

AU - Cho, Woo Jin

AU - Lee, Jae Yong

PY - 2005/8/5

Y1 - 2005/8/5

N2 - Conclusions. Our results indicate that the electrogenic kidney Na +/HCO 3 cotransporter (kNBC), KCl cotransporter (KCC1 and -4) and Ca 2+-activated Cl - channel (CaCC1, -2, -3) mRNAs are expressed in normal nasal mucosa and nasal polyp, suggesting that altered expression of all CaCC mRNAs in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells. Objective. Electrolyte transport by nasal epithelia has been suggested to be important for controlling the quantity and composition of the nasal fluid and may play an important role in the development of nasal polyps. Transepithelial transport of ions and water in various fluid-transporting epithelia is strictly dependent on the localization of specific membrane proteins in the polarized epithelial cells. In this study we investigated the expression and distribution of mRNA transcripts for kNBC, pancreatic NBC, KCC1, -2, -3, -4 and CaCC1, -2, -3 gene families in human nasal mucosa and nasal polyp. Material and methods. The expression and localization of these gene families were investigated in inferior turbinate tissues and nasal polyp using reverse transcriptase polymerase chain reaction (RT-PCR), semiquantitative RT-PCR and in situ hybridization. Results. mRNAs for kNBC, KCC1 and -4 and all the CaCC families (CaCC1, -2 and -3) are expressed in human turbinate mucosa and nasal polyp. The expression levels of kNBC and KCC1 and -4 mRNAs did not differ between nasal mucosa and nasal polyp. However, the expression levels of all the CaCC genes were significantly decreased in nasal polyp. In situ hybridization revealed that the expression of these genes was mainly localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyp.

AB - Conclusions. Our results indicate that the electrogenic kidney Na +/HCO 3 cotransporter (kNBC), KCl cotransporter (KCC1 and -4) and Ca 2+-activated Cl - channel (CaCC1, -2, -3) mRNAs are expressed in normal nasal mucosa and nasal polyp, suggesting that altered expression of all CaCC mRNAs in nasal polyp may cause impaired electrolyte and water transport across the epithelial cells. Objective. Electrolyte transport by nasal epithelia has been suggested to be important for controlling the quantity and composition of the nasal fluid and may play an important role in the development of nasal polyps. Transepithelial transport of ions and water in various fluid-transporting epithelia is strictly dependent on the localization of specific membrane proteins in the polarized epithelial cells. In this study we investigated the expression and distribution of mRNA transcripts for kNBC, pancreatic NBC, KCC1, -2, -3, -4 and CaCC1, -2, -3 gene families in human nasal mucosa and nasal polyp. Material and methods. The expression and localization of these gene families were investigated in inferior turbinate tissues and nasal polyp using reverse transcriptase polymerase chain reaction (RT-PCR), semiquantitative RT-PCR and in situ hybridization. Results. mRNAs for kNBC, KCC1 and -4 and all the CaCC families (CaCC1, -2 and -3) are expressed in human turbinate mucosa and nasal polyp. The expression levels of kNBC and KCC1 and -4 mRNAs did not differ between nasal mucosa and nasal polyp. However, the expression levels of all the CaCC genes were significantly decreased in nasal polyp. In situ hybridization revealed that the expression of these genes was mainly localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyp.

KW - Ca -activated channel

KW - In situ hybridization

KW - KCl cotransporter

KW - Na /HCO cotransporter

KW - Reverse transcriptase polymerase chain reaction

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