Expression and mutation analyses of p53R2, a newly identified p53 target for dna repair in human gastric carcinoma

Do Sun Byun, Kwon Seok Chae, Byung Kyu Ryu, Min Goo Lee, Sung-Gil Chi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

p53R2, a recently identified putative tumor suppressor located at 8q23.1, encodes a protein with striking similarity to a small subunit of ribonucleotide reductase. p53R2 is directly induced by wild-type p53 and involved in the p53 checkpoint for repair of damaged DNA, raising the possibility that mutational inactivation of p53R2 may contribute to the development and progression of human malignancies. To explore the p53R2's candidacy for a suppressor in gastric tumorigenesis, we examined the expression and mutation status of p53R2 in 166 gastric specimens including 90 primary adenocarcinomas and 15 cell lines. In response to genotoxic damages, p53R2 transcription was clearly activated in wild-type but not mutant-type p53-carrying cells while basal expression of p53R2 in undamaged cells showed no association with the mutational status of p53. Host cell reactivation assay revealed that p53R2 enhances DNA repair efficiency and plays a role in the p53-mediated repair of damaged DNA, whereas no significant effect of p53R2 on cell growth and apoptosis was detected in flow cytometry and [3H]thymidine incorporation assays. p53R2 transcript was expressed in all normal and tumor tissues and its expression levels were not significantly different between normal and malignant carcinoma tissues. p53R2 expression showed no correlation with stage, grade and histological types of tumors. Moreover, no tumor-specific reduction of p53R2 was detected in 30 matched sets. Mutational analysis of p53R2 in 105 carcinomas including 15 cell lines also failed to detect any evidences for genomic deletion or somatic mutations leading to amino acid substitutions or frameshift whereas 31% (28 of 90) of the same primary tumors showed p53 alterations. Whereas 82% (23 of 28) of the mutant p53-carrying primary tumors expressed abnormally low p21Wafl, no association of p53R2 expression with the p53 status was recognized, suggesting that basal transcription of p53R2 is regulated through the p53-independent mechanism. Collectively, our study indicates that although p53R2 is induced in a p53-dependent manner and involved the p53-mediated DNA repair in gastric epithelial cells, it is not a critical target of genetic inactivation in gastric tumorigenesis.

Original languageEnglish
Pages (from-to)718-723
Number of pages6
JournalInternational Journal of Cancer
Volume98
Issue number5
DOIs
Publication statusPublished - 2002 Apr 10
Externally publishedYes

Fingerprint

Stomach
Carcinoma
Mutation
DNA Repair
Neoplasms
Carcinogenesis
Ribonucleotide Reductases
Cell Line
Human Development
Amino Acid Substitution
Thymidine
Flow Cytometry
Adenocarcinoma
Epithelial Cells
Apoptosis
Growth
Proteins

Keywords

  • DNA repair
  • Gastric cancer
  • p53
  • p53R2

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Expression and mutation analyses of p53R2, a newly identified p53 target for dna repair in human gastric carcinoma. / Byun, Do Sun; Chae, Kwon Seok; Ryu, Byung Kyu; Lee, Min Goo; Chi, Sung-Gil.

In: International Journal of Cancer, Vol. 98, No. 5, 10.04.2002, p. 718-723.

Research output: Contribution to journalArticle

Byun, Do Sun ; Chae, Kwon Seok ; Ryu, Byung Kyu ; Lee, Min Goo ; Chi, Sung-Gil. / Expression and mutation analyses of p53R2, a newly identified p53 target for dna repair in human gastric carcinoma. In: International Journal of Cancer. 2002 ; Vol. 98, No. 5. pp. 718-723.
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