Expression and mutational analysis of TGF-β/Smads signaling in human cervical cancers

Kyung Do Ki, Seo Yun Tong, Chu Yeop Huh, Jong Min Lee, Seon Kyung Lee, Sung Gil Chi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective: To define the molecular basis of TGF-β1 function in cervical carcinogenesis, we explored the expression and mutational status of TGF-β1, TGF-β1 receptors, and Smads, the regulators of the TGF-β1 signaling pathway, in human cervical cancers. Methods: Expression of TGF-β1, TGF-β1 receptors, and Smads transcripts were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR), and sequence alteration was analyzed using RT-PCR-single-strand conformation polymorphism (SSCP) analysis. Genomic levels of TGF-β1, TGF-β1 receptors and Smads was also measured by quantitative genomic PCR. Results: Abnormal overexpression of TGF-β1 and abnormal reduction of type II TGF-β1 receptor were identified in 36% (18 of 50) and 20% (10 of 50) of cervical cancer tissues, respectively. 22% (11 of 50) in Smad2 and 14% (7 of 50) in Smad4 revealed tumor specific mRNA reduction less than a half of normal means. In addition, no evidence for sequence alterations of the gene was found by RT-PCR-SSCP analysis. Conclusion: Our study demonstrates that disruption of TGF-β/Smad signaling pathway exist in human cervical cancer, suggesting that abnormal expressions of the member of TGF-β/Smad signaling pathway might contribute to the malignant progression of human cervical tumors via suppressing the tumor suppression function of TGF-β1 1's tumor suppression function.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalJournal of gynecologic oncology
Volume20
Issue number2
DOIs
Publication statusPublished - 2009 Jun

Keywords

  • Cervical cancer
  • Expression
  • TGF-β/Smads

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

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