Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.)

Cheol Seong Jang, Hojoung Lee, Suck Joo Chang, Yong Weon Seo

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

In a previous report, a gene encoding lipid transfer protein (LTP), TaLTP1, was characterized as induced by dehydration in a 2BS/2RL wheat-rye translocation. For the further study of this gene, gene expression and promoter analysis under water stress treatments were performed. Transcripts of the TaLTP1 gene were increased by water stresses, such as by treatment at several PEG concentrations and NaCl, by hormone treatments (SA, ethephon), by H 2O2, or by wounding. The TaLTP1 gene did not contain any intron sequences as compared with its cDNA and genomic DNA sequence. Sequence analysis of the TaLTP1 promoter was performed from the 2856 bp upstream of the transcription start site. A series of five 5′ deletions of the TaLTP1 gene promoter were amplified with each of five upstream primers and one downstream primer and fused with GUS reporter gene on the pBI101 binary vector. All of the five deletions showed similar GUS activity, which was higher than those of mock treatment during drought or salt treatment. These results showed that 337 bp DNA fragment of the upstream region have drought and salt stress-responsive elements. The 337 bp fragment region contained five putative MYC-like and one MYB-like sequences. Therefore, cis-elements located in the 337 bp DNA fragment within promoter region may control the transcription of the TaLTP1 gene during dehydration and periods of salt stress in wheat.

Original languageEnglish
Pages (from-to)995-1001
Number of pages7
JournalPlant Science
Volume167
Issue number5
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Drought
Droughts
Triticum
salt stress
water stress
Dehydration
Triticum aestivum
Salts
Genes
promoter regions
wheat
dehydration (animal physiology)
genes
transcription (genetics)
gene deletion
Transcription Initiation Site
DNA
ethephon
Gene Deletion
Gene encoding

Keywords

  • MYB
  • MYC
  • Promoter
  • TaLTP1 gene
  • Water stress

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.). / Jang, Cheol Seong; Lee, Hojoung; Chang, Suck Joo; Seo, Yong Weon.

In: Plant Science, Vol. 167, No. 5, 01.11.2004, p. 995-1001.

Research output: Contribution to journalArticle

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