Expression and regulation of SNAP-25 and synaptotagmin VII in developing mouse ovarian follicles via the FSH receptor

Sung Sik Choi, Joo Young Jung, Dongho Lee, Ji Yoon Kang, Sang Ho Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Soluble-NSF attachment protein receptor (SNARE) proteins play a role in vesicle fusion, exocytosis, and intracellular trafficking in neuronal cells as well as in fertilization and embryogenesis. We investigated the expression patterns of two SNARE proteins, SNAP-25 and synaptotagmin VII (SytVII), and their regulation by pregnant mare serum gonadotropin (PMSG) during mouse ovarian follicular development. Ovaries were obtained at 0, 12, 24, 36, and 48 h post-PMSG injection of immature mice. SNAP-25 and SytVII mRNA expression levels increased gradually in a time-dependant manner. However, protein levels revealed different patterns of expression, suggesting different translational regulation following PMSG stimulation. SNAP-25 and SytVII expression was closely associated with thickening of the granulosa cell (GC) layer and follicle morphological changes from a flattened to a cuboidal shape. To explore follicle stimulating hormone receptor (FSHR)-mediated regulation of their expression, GCs from preantral follicles were cultured to examine the effects of FSHR siRNA knockdown. FSHR siRNA abolished upregulation of the SNAREs in both PMSG and FSH-stimulated GCs. This abolished gene expression was rescued by adding dibutyryl cyclic AMP to the cultures. These results suggest that SNAP-25 and SytVII expression is regulated via the FSHR-cAMP pathway during follicular development.

Original languageEnglish
Pages (from-to)47-54
Number of pages8
JournalJournal of Molecular Histology
Volume44
Issue number1
DOIs
Publication statusPublished - 2013 Feb 1

Fingerprint

Synaptotagmins
FSH Receptors
Equine Gonadotropins
Ovarian Follicle
SNARE Proteins
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Small Interfering RNA
Synaptosomal-Associated Protein 25
Bucladesine
Granulosa Cells
Exocytosis
Fertilization
Embryonic Development
Ovary
Proteins
Up-Regulation
Gene Expression
Messenger RNA
Injections

Keywords

  • cAMP
  • Follicle
  • FSH receptor
  • Granulosa cells
  • SNAREs

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Physiology

Cite this

Expression and regulation of SNAP-25 and synaptotagmin VII in developing mouse ovarian follicles via the FSH receptor. / Choi, Sung Sik; Jung, Joo Young; Lee, Dongho; Kang, Ji Yoon; Lee, Sang Ho.

In: Journal of Molecular Histology, Vol. 44, No. 1, 01.02.2013, p. 47-54.

Research output: Contribution to journalArticle

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