Abstract
Thymosin β15 (Tβ15) is a pleiotropic factor which exerts multiple roles in the development of nervous system and brain diseases. In this study, we found that the expressions of Tβ15 mRNA and protein were substantially increased in several brain regions including hippocampal formation and cerebral cortex, following kainic acid (KA)-evoked seizures in rat. Interestingly, a subset of cortex neurons exhibited nuclear Tβ15 immunoreactivity upon KA treatment. Furthermore, translocation of Tβ15 from cytosol to nuclei was observed in cultured neurons or HeLa cells during staurosporine (STS)-induced apoptosis, which was also verified by time-lapse imaging of YFP-tagged Tβ15. It appeared that localization of Tβ15 is restricted to the cytosol in normal condition by its G-actin-interacting domain, because site-directed mutagenesis of this region resulted in the nuclear localization of Tβ15 in the absence of STS treatment. To explore the role of nuclear Tβ15, we enforced Tβ15 to localize in the nuclei by fusion of Tβ15 with nuclear localization signal (NLS-Tβ15). However, overexpression of NLS-Tβ15 did not alter the viability of cells in response to STS treatment. Collectively, these results suggest that nuclear localization of Tβ15 is a controlled process during KA or STS stimulation, although its functional significance is yet to be clarified.
| Original language | English |
|---|---|
| Pages (from-to) | 664-669 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 371 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 Jul 11 |
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Keywords
- Kainic acid
- Nuclear translocation
- Staurosporine-induced apoptosis
- Thymosin β15
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Expression and subcellular localization of thymosin beta15 following kainic acid treatment in rat brain. / Kim, Young Woong; Kim, Younghwa; Kim, Eun Hae; Koh, Doyle; Sun, Woong; Kim, Hyun.
In: Biochemical and Biophysical Research Communications, Vol. 371, No. 4, 11.07.2008, p. 664-669.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and subcellular localization of thymosin beta15 following kainic acid treatment in rat brain
AU - Kim, Young Woong
AU - Kim, Younghwa
AU - Kim, Eun Hae
AU - Koh, Doyle
AU - Sun, Woong
AU - Kim, Hyun
PY - 2008/7/11
Y1 - 2008/7/11
N2 - Thymosin β15 (Tβ15) is a pleiotropic factor which exerts multiple roles in the development of nervous system and brain diseases. In this study, we found that the expressions of Tβ15 mRNA and protein were substantially increased in several brain regions including hippocampal formation and cerebral cortex, following kainic acid (KA)-evoked seizures in rat. Interestingly, a subset of cortex neurons exhibited nuclear Tβ15 immunoreactivity upon KA treatment. Furthermore, translocation of Tβ15 from cytosol to nuclei was observed in cultured neurons or HeLa cells during staurosporine (STS)-induced apoptosis, which was also verified by time-lapse imaging of YFP-tagged Tβ15. It appeared that localization of Tβ15 is restricted to the cytosol in normal condition by its G-actin-interacting domain, because site-directed mutagenesis of this region resulted in the nuclear localization of Tβ15 in the absence of STS treatment. To explore the role of nuclear Tβ15, we enforced Tβ15 to localize in the nuclei by fusion of Tβ15 with nuclear localization signal (NLS-Tβ15). However, overexpression of NLS-Tβ15 did not alter the viability of cells in response to STS treatment. Collectively, these results suggest that nuclear localization of Tβ15 is a controlled process during KA or STS stimulation, although its functional significance is yet to be clarified.
AB - Thymosin β15 (Tβ15) is a pleiotropic factor which exerts multiple roles in the development of nervous system and brain diseases. In this study, we found that the expressions of Tβ15 mRNA and protein were substantially increased in several brain regions including hippocampal formation and cerebral cortex, following kainic acid (KA)-evoked seizures in rat. Interestingly, a subset of cortex neurons exhibited nuclear Tβ15 immunoreactivity upon KA treatment. Furthermore, translocation of Tβ15 from cytosol to nuclei was observed in cultured neurons or HeLa cells during staurosporine (STS)-induced apoptosis, which was also verified by time-lapse imaging of YFP-tagged Tβ15. It appeared that localization of Tβ15 is restricted to the cytosol in normal condition by its G-actin-interacting domain, because site-directed mutagenesis of this region resulted in the nuclear localization of Tβ15 in the absence of STS treatment. To explore the role of nuclear Tβ15, we enforced Tβ15 to localize in the nuclei by fusion of Tβ15 with nuclear localization signal (NLS-Tβ15). However, overexpression of NLS-Tβ15 did not alter the viability of cells in response to STS treatment. Collectively, these results suggest that nuclear localization of Tβ15 is a controlled process during KA or STS stimulation, although its functional significance is yet to be clarified.
KW - Kainic acid
KW - Nuclear translocation
KW - Staurosporine-induced apoptosis
KW - Thymosin β15
UR - http://www.scopus.com/inward/record.url?scp=44349122293&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44349122293&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.04.112
DO - 10.1016/j.bbrc.2008.04.112
M3 - Article
VL - 371
SP - 664
EP - 669
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 4
ER -