Expression and subcellular localization of thymosin beta15 following kainic acid treatment in rat brain

Young Woong Kim, Younghwa Kim, Eun Hae Kim, Doyle Koh, Woong Sun, Hyun Kim

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4 Citations (Scopus)

Abstract

Thymosin β15 (Tβ15) is a pleiotropic factor which exerts multiple roles in the development of nervous system and brain diseases. In this study, we found that the expressions of Tβ15 mRNA and protein were substantially increased in several brain regions including hippocampal formation and cerebral cortex, following kainic acid (KA)-evoked seizures in rat. Interestingly, a subset of cortex neurons exhibited nuclear Tβ15 immunoreactivity upon KA treatment. Furthermore, translocation of Tβ15 from cytosol to nuclei was observed in cultured neurons or HeLa cells during staurosporine (STS)-induced apoptosis, which was also verified by time-lapse imaging of YFP-tagged Tβ15. It appeared that localization of Tβ15 is restricted to the cytosol in normal condition by its G-actin-interacting domain, because site-directed mutagenesis of this region resulted in the nuclear localization of Tβ15 in the absence of STS treatment. To explore the role of nuclear Tβ15, we enforced Tβ15 to localize in the nuclei by fusion of Tβ15 with nuclear localization signal (NLS-Tβ15). However, overexpression of NLS-Tβ15 did not alter the viability of cells in response to STS treatment. Collectively, these results suggest that nuclear localization of Tβ15 is a controlled process during KA or STS stimulation, although its functional significance is yet to be clarified.

Original languageEnglish
Pages (from-to)664-669
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume371
Issue number4
DOIs
Publication statusPublished - 2008 Jul 11

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Keywords

  • Kainic acid
  • Nuclear translocation
  • Staurosporine-induced apoptosis
  • Thymosin β15

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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