Expression levels of endogenous hydrogen sulfide are altered in patients with allergic rhinitis

Se Jin Park, Tae-Hoon Kim, Seung Hoon Lee, Hyei Yul Ryu, Ki Hong Hong, Jong Yoon Jung, Gyu Ho Hwang, Sang Hag Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives/Hypothesis: Hydrogen sulfide (H2S), the third endogenous gaseous transmitter, may be a crucial mediator in airway hyper-responsiveness and airway inflammation, including asthma. To elucidate the role of H2S in allergic rhinitis, the present study was undertaken to determine the level of expression of H2S in healthy nasal mucosa and mild and moderate/severe persistent allergic nasal mucosa as well as peripheral blood obtained from each patient. The expression and distribution pattern of the H2S-synthesizing enzymes cystathione γ-lyase (CSE) and cystathione β-synthase (CBS) were investigated in healthy and allergic nasal mucosa. Study Design: Controlled, prospective study. Methods: The concentration of H2S in nasal mucosa and plasma was determined by zinc trap spectrophotometry. The expression levels and patterns of distribution of CSE and CBS mRNA and proteins were evaluated using real time polymerase chain reaction, Western blot, and immunohistochemistry. Results: The levels of expression of H2S in nasal mucosa and plasma were increased in patients with mild and moderate/severe persistent allergic rhinitis compared with healthy controls. CSE was localized in vascular endothelium and surrounding muscles, and submucosal glands, whereas CBS was exclusively distributed in the superficial epithelium and submucosal glands. Their expression levels were increased in mild and moderate/severe persistent allergic rhinitis. Conclusions: The current findings indicate that, in parallel with increased expression levels of CSE and CBS, H2S is upregulated in nasal mucosa and plasma of allergic patients. Based on localization of CSE and CBS, H2S may play multiple functions in human nasal mucosa, contributing to the development of allergic symptoms such as rhinorrhea, sneezing, and nasal stuffiness. Laryngoscope, 2013

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalLaryngoscope
Volume123
Issue number3
DOIs
Publication statusPublished - 2013 Mar 1

Fingerprint

Hydrogen Sulfide
Nasal Mucosa
Lyases
Respiratory Hypersensitivity
Sneezing
Laryngoscopes
Spectrophotometry
Vascular Endothelium
Allergic Rhinitis
Nose
Zinc
Real-Time Polymerase Chain Reaction
Asthma
Epithelium
Western Blotting
Immunohistochemistry
Prospective Studies
Inflammation
Muscles
Messenger RNA

Keywords

  • cystathione β-synthase
  • Cystathione γ-lyase
  • hydrogen sulfide
  • Level of Evidence: 2c
  • mild persistent allergic rhinitis
  • moderate/severe persistent allergic rhinitis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression levels of endogenous hydrogen sulfide are altered in patients with allergic rhinitis. / Park, Se Jin; Kim, Tae-Hoon; Lee, Seung Hoon; Ryu, Hyei Yul; Hong, Ki Hong; Jung, Jong Yoon; Hwang, Gyu Ho; Lee, Sang Hag.

In: Laryngoscope, Vol. 123, No. 3, 01.03.2013, p. 557-563.

Research output: Contribution to journalArticle

Park, Se Jin ; Kim, Tae-Hoon ; Lee, Seung Hoon ; Ryu, Hyei Yul ; Hong, Ki Hong ; Jung, Jong Yoon ; Hwang, Gyu Ho ; Lee, Sang Hag. / Expression levels of endogenous hydrogen sulfide are altered in patients with allergic rhinitis. In: Laryngoscope. 2013 ; Vol. 123, No. 3. pp. 557-563.
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abstract = "Objectives/Hypothesis: Hydrogen sulfide (H2S), the third endogenous gaseous transmitter, may be a crucial mediator in airway hyper-responsiveness and airway inflammation, including asthma. To elucidate the role of H2S in allergic rhinitis, the present study was undertaken to determine the level of expression of H2S in healthy nasal mucosa and mild and moderate/severe persistent allergic nasal mucosa as well as peripheral blood obtained from each patient. The expression and distribution pattern of the H2S-synthesizing enzymes cystathione γ-lyase (CSE) and cystathione β-synthase (CBS) were investigated in healthy and allergic nasal mucosa. Study Design: Controlled, prospective study. Methods: The concentration of H2S in nasal mucosa and plasma was determined by zinc trap spectrophotometry. The expression levels and patterns of distribution of CSE and CBS mRNA and proteins were evaluated using real time polymerase chain reaction, Western blot, and immunohistochemistry. Results: The levels of expression of H2S in nasal mucosa and plasma were increased in patients with mild and moderate/severe persistent allergic rhinitis compared with healthy controls. CSE was localized in vascular endothelium and surrounding muscles, and submucosal glands, whereas CBS was exclusively distributed in the superficial epithelium and submucosal glands. Their expression levels were increased in mild and moderate/severe persistent allergic rhinitis. Conclusions: The current findings indicate that, in parallel with increased expression levels of CSE and CBS, H2S is upregulated in nasal mucosa and plasma of allergic patients. Based on localization of CSE and CBS, H2S may play multiple functions in human nasal mucosa, contributing to the development of allergic symptoms such as rhinorrhea, sneezing, and nasal stuffiness. Laryngoscope, 2013",
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AU - Kim, Tae-Hoon

AU - Lee, Seung Hoon

AU - Ryu, Hyei Yul

AU - Hong, Ki Hong

AU - Jung, Jong Yoon

AU - Hwang, Gyu Ho

AU - Lee, Sang Hag

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AB - Objectives/Hypothesis: Hydrogen sulfide (H2S), the third endogenous gaseous transmitter, may be a crucial mediator in airway hyper-responsiveness and airway inflammation, including asthma. To elucidate the role of H2S in allergic rhinitis, the present study was undertaken to determine the level of expression of H2S in healthy nasal mucosa and mild and moderate/severe persistent allergic nasal mucosa as well as peripheral blood obtained from each patient. The expression and distribution pattern of the H2S-synthesizing enzymes cystathione γ-lyase (CSE) and cystathione β-synthase (CBS) were investigated in healthy and allergic nasal mucosa. Study Design: Controlled, prospective study. Methods: The concentration of H2S in nasal mucosa and plasma was determined by zinc trap spectrophotometry. The expression levels and patterns of distribution of CSE and CBS mRNA and proteins were evaluated using real time polymerase chain reaction, Western blot, and immunohistochemistry. Results: The levels of expression of H2S in nasal mucosa and plasma were increased in patients with mild and moderate/severe persistent allergic rhinitis compared with healthy controls. CSE was localized in vascular endothelium and surrounding muscles, and submucosal glands, whereas CBS was exclusively distributed in the superficial epithelium and submucosal glands. Their expression levels were increased in mild and moderate/severe persistent allergic rhinitis. Conclusions: The current findings indicate that, in parallel with increased expression levels of CSE and CBS, H2S is upregulated in nasal mucosa and plasma of allergic patients. Based on localization of CSE and CBS, H2S may play multiple functions in human nasal mucosa, contributing to the development of allergic symptoms such as rhinorrhea, sneezing, and nasal stuffiness. Laryngoscope, 2013

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KW - Cystathione γ-lyase

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KW - Level of Evidence: 2c

KW - mild persistent allergic rhinitis

KW - moderate/severe persistent allergic rhinitis

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