TY - JOUR
T1 - Expression of β-defensins in the tubotympanum of experimental otitis media
AU - Shin, Dong Jin
AU - Gan-Undram, Semeibsuren
AU - Kim, Seo Jin
AU - Jun, Young Joon
AU - Im, Gi Jung
AU - Jung, Hak Hyun
N1 - Funding Information:
This study was supported by the Brain Korea Project 21 and by the Institutes of Communication Disorder of Korea University.
PY - 2006/10/1
Y1 - 2006/10/1
N2 - Conclusion. Since the expression levels of β-defensins 2-4 were up-regulated in experimental otitis media, they may play a protective role in the pathogenesis of otitis media. Objectives. Defensins are antimicrobial peptides that play a major role in innate immunity. The goal of this study was to identify the expression of defensins in experimental otitis media of the mouse. Materials and methods. The expression of three α-defensins (cryptdins, cryptdin-related sequences 1-C (CRS1-C), and CRS4-C) and four β-defensins (mBD1, mBD2, mBD3, and mBD4) was investigated in the tubotympanum of experimental otitis media in mice by a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the expression levels of three β-defensins (mBD2, mBD3, mBD4) were evaluated by Western blotting. Results. The α-defensins were not expressed in the tubotympanum. mBD1 was expressed constitutively in normal middle ear mucosa and Eustachian tube mucosa, but up-regulated expression of mBD2, mBD3, and mBD4 was observed with RT-PCR and Western blotting in the tubotympanums in experimental otitis media, while the normal tubotympanums did not express them.
AB - Conclusion. Since the expression levels of β-defensins 2-4 were up-regulated in experimental otitis media, they may play a protective role in the pathogenesis of otitis media. Objectives. Defensins are antimicrobial peptides that play a major role in innate immunity. The goal of this study was to identify the expression of defensins in experimental otitis media of the mouse. Materials and methods. The expression of three α-defensins (cryptdins, cryptdin-related sequences 1-C (CRS1-C), and CRS4-C) and four β-defensins (mBD1, mBD2, mBD3, and mBD4) was investigated in the tubotympanum of experimental otitis media in mice by a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the expression levels of three β-defensins (mBD2, mBD3, mBD4) were evaluated by Western blotting. Results. The α-defensins were not expressed in the tubotympanum. mBD1 was expressed constitutively in normal middle ear mucosa and Eustachian tube mucosa, but up-regulated expression of mBD2, mBD3, and mBD4 was observed with RT-PCR and Western blotting in the tubotympanums in experimental otitis media, while the normal tubotympanums did not express them.
KW - Gene
KW - Mouse
KW - Otitis media
KW - Pathogenesis
UR - http://www.scopus.com/inward/record.url?scp=33747727656&partnerID=8YFLogxK
U2 - 10.1080/00016480600672626
DO - 10.1080/00016480600672626
M3 - Article
C2 - 16923707
AN - SCOPUS:33747727656
SN - 0001-6489
VL - 126
SP - 1040
EP - 1045
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
IS - 10
ER -