Expression of β-defensins in the tubotympanum of experimental otitis media

Dong Jin Shin, Semeibsuren Gan-Undram, Seo Jin Kim, Young Joon Jun, Gi Jung Im, Hak Hyun Jung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Conclusion. Since the expression levels of β-defensins 2-4 were up-regulated in experimental otitis media, they may play a protective role in the pathogenesis of otitis media. Objectives. Defensins are antimicrobial peptides that play a major role in innate immunity. The goal of this study was to identify the expression of defensins in experimental otitis media of the mouse. Materials and methods. The expression of three α-defensins (cryptdins, cryptdin-related sequences 1-C (CRS1-C), and CRS4-C) and four β-defensins (mBD1, mBD2, mBD3, and mBD4) was investigated in the tubotympanum of experimental otitis media in mice by a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the expression levels of three β-defensins (mBD2, mBD3, mBD4) were evaluated by Western blotting. Results. The α-defensins were not expressed in the tubotympanum. mBD1 was expressed constitutively in normal middle ear mucosa and Eustachian tube mucosa, but up-regulated expression of mBD2, mBD3, and mBD4 was observed with RT-PCR and Western blotting in the tubotympanums in experimental otitis media, while the normal tubotympanums did not express them.

Original languageEnglish
Pages (from-to)1040-1045
Number of pages6
JournalActa Oto-Laryngologica
Volume126
Issue number10
DOIs
Publication statusPublished - 2006 Oct 1

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Defensins
Otitis Media
Reverse Transcription
Mucous Membrane
Western Blotting
Eustachian Tube
Polymerase Chain Reaction
Middle Ear
Innate Immunity
Peptides

Keywords

  • Gene
  • Mouse
  • Otitis media
  • Pathogenesis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression of β-defensins in the tubotympanum of experimental otitis media. / Shin, Dong Jin; Gan-Undram, Semeibsuren; Kim, Seo Jin; Jun, Young Joon; Im, Gi Jung; Jung, Hak Hyun.

In: Acta Oto-Laryngologica, Vol. 126, No. 10, 01.10.2006, p. 1040-1045.

Research output: Contribution to journalArticle

Shin, Dong Jin ; Gan-Undram, Semeibsuren ; Kim, Seo Jin ; Jun, Young Joon ; Im, Gi Jung ; Jung, Hak Hyun. / Expression of β-defensins in the tubotympanum of experimental otitis media. In: Acta Oto-Laryngologica. 2006 ; Vol. 126, No. 10. pp. 1040-1045.
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