Expression of a Cathelicidin antimicrobial peptide is augmented in cholesteatoma

Hak Hyun Jung, Sungwon Chae, Seol Ki Jung, Seon Tae, Kim Heung Man Lee, Soon Jae Hwang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objectives/Hypothesis: Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated in cholesteatoma epithelium compared with normal skin. Study Design: Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for immunohistochemical staining. Methods: Reverse transcriptase-polymerase chain reaction was used to assess the expression levels of cathelicidin messenger RNA (mRNA) both in cholesteatoma and in normal skin. Presumptive concentration of cathelicidin mRNA and β2-microglobulin mRNA was evaluated. Ratio of β2-microglobulin to cathelicidin was analyzed in each group. The expressions of cathelicidin in cholesteatoma and normal skin epithelium were investigated by an immunohistochemical technique. Results: Cathelicidin mRNA in cholesteatoma epithelium was increased 5.5-fold compared with normal skin of the ear canal. In cholesteatoma epithelium, cathelicidin was located in all the layers, but in the normal skin it was expressed only in the granular and prickle cell layers. Conclusions: Cathelicidin is augmented in cholesteatoma epithelium, and the data in the present study are in agreement with the hypothesis that cathelicidin is likely to act as a key component in the first line of defense at the surface epithelium.

Original languageEnglish
Pages (from-to)432-435
Number of pages4
JournalLaryngoscope
Volume113
Issue number3
DOIs
Publication statusPublished - 2003 Mar 1

Fingerprint

Cholesteatoma
Epithelium
Skin
Messenger RNA
Cathelicidins
CAP18 lipopolysaccharide-binding protein
cathelicidin antimicrobial peptide
Peptides
Ear Canal
Infection
Reverse Transcriptase Polymerase Chain Reaction
Bacterial Infections
Innate Immunity
Prospective Studies
RNA
Staining and Labeling
Anti-Bacterial Agents

Keywords

  • Cathelicidin
  • Cholesteatoma
  • Immunohistochemical staining
  • Reverse transcriptase-polymerase chain reaction

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression of a Cathelicidin antimicrobial peptide is augmented in cholesteatoma. / Jung, Hak Hyun; Chae, Sungwon; Jung, Seol Ki; Tae, Seon; Lee, Kim Heung Man; Hwang, Soon Jae.

In: Laryngoscope, Vol. 113, No. 3, 01.03.2003, p. 432-435.

Research output: Contribution to journalArticle

Jung, Hak Hyun ; Chae, Sungwon ; Jung, Seol Ki ; Tae, Seon ; Lee, Kim Heung Man ; Hwang, Soon Jae. / Expression of a Cathelicidin antimicrobial peptide is augmented in cholesteatoma. In: Laryngoscope. 2003 ; Vol. 113, No. 3. pp. 432-435.
@article{2f7fb8609c894bf8b3cfe3b76e10a65e,
title = "Expression of a Cathelicidin antimicrobial peptide is augmented in cholesteatoma",
abstract = "Objectives/Hypothesis: Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated in cholesteatoma epithelium compared with normal skin. Study Design: Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for immunohistochemical staining. Methods: Reverse transcriptase-polymerase chain reaction was used to assess the expression levels of cathelicidin messenger RNA (mRNA) both in cholesteatoma and in normal skin. Presumptive concentration of cathelicidin mRNA and β2-microglobulin mRNA was evaluated. Ratio of β2-microglobulin to cathelicidin was analyzed in each group. The expressions of cathelicidin in cholesteatoma and normal skin epithelium were investigated by an immunohistochemical technique. Results: Cathelicidin mRNA in cholesteatoma epithelium was increased 5.5-fold compared with normal skin of the ear canal. In cholesteatoma epithelium, cathelicidin was located in all the layers, but in the normal skin it was expressed only in the granular and prickle cell layers. Conclusions: Cathelicidin is augmented in cholesteatoma epithelium, and the data in the present study are in agreement with the hypothesis that cathelicidin is likely to act as a key component in the first line of defense at the surface epithelium.",
keywords = "Cathelicidin, Cholesteatoma, Immunohistochemical staining, Reverse transcriptase-polymerase chain reaction",
author = "Jung, {Hak Hyun} and Sungwon Chae and Jung, {Seol Ki} and Seon Tae and Lee, {Kim Heung Man} and Hwang, {Soon Jae}",
year = "2003",
month = "3",
day = "1",
doi = "10.1097/00005537-200303000-00008",
language = "English",
volume = "113",
pages = "432--435",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Expression of a Cathelicidin antimicrobial peptide is augmented in cholesteatoma

AU - Jung, Hak Hyun

AU - Chae, Sungwon

AU - Jung, Seol Ki

AU - Tae, Seon

AU - Lee, Kim Heung Man

AU - Hwang, Soon Jae

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Objectives/Hypothesis: Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated in cholesteatoma epithelium compared with normal skin. Study Design: Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for immunohistochemical staining. Methods: Reverse transcriptase-polymerase chain reaction was used to assess the expression levels of cathelicidin messenger RNA (mRNA) both in cholesteatoma and in normal skin. Presumptive concentration of cathelicidin mRNA and β2-microglobulin mRNA was evaluated. Ratio of β2-microglobulin to cathelicidin was analyzed in each group. The expressions of cathelicidin in cholesteatoma and normal skin epithelium were investigated by an immunohistochemical technique. Results: Cathelicidin mRNA in cholesteatoma epithelium was increased 5.5-fold compared with normal skin of the ear canal. In cholesteatoma epithelium, cathelicidin was located in all the layers, but in the normal skin it was expressed only in the granular and prickle cell layers. Conclusions: Cathelicidin is augmented in cholesteatoma epithelium, and the data in the present study are in agreement with the hypothesis that cathelicidin is likely to act as a key component in the first line of defense at the surface epithelium.

AB - Objectives/Hypothesis: Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated in cholesteatoma epithelium compared with normal skin. Study Design: Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for immunohistochemical staining. Methods: Reverse transcriptase-polymerase chain reaction was used to assess the expression levels of cathelicidin messenger RNA (mRNA) both in cholesteatoma and in normal skin. Presumptive concentration of cathelicidin mRNA and β2-microglobulin mRNA was evaluated. Ratio of β2-microglobulin to cathelicidin was analyzed in each group. The expressions of cathelicidin in cholesteatoma and normal skin epithelium were investigated by an immunohistochemical technique. Results: Cathelicidin mRNA in cholesteatoma epithelium was increased 5.5-fold compared with normal skin of the ear canal. In cholesteatoma epithelium, cathelicidin was located in all the layers, but in the normal skin it was expressed only in the granular and prickle cell layers. Conclusions: Cathelicidin is augmented in cholesteatoma epithelium, and the data in the present study are in agreement with the hypothesis that cathelicidin is likely to act as a key component in the first line of defense at the surface epithelium.

KW - Cathelicidin

KW - Cholesteatoma

KW - Immunohistochemical staining

KW - Reverse transcriptase-polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0037337850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037337850&partnerID=8YFLogxK

U2 - 10.1097/00005537-200303000-00008

DO - 10.1097/00005537-200303000-00008

M3 - Article

VL - 113

SP - 432

EP - 435

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 3

ER -