Expression of an expansin gene is correlated with root elongation in soybean

Dong Keun Lee, Ji Hoon Ahn, Sang Kee Song, Yang Do Choi, Jong Seob Lee

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Expansin is a family of proteins that catalyze long-term expansion of cell walls and has been considered a principal protein that affects cell expansion in plants. We have identified the first root-specific expansin gene in soybean (Glycine max), GmEXP1, which may be responsible for root elongation. Expression levels of GmEXP1 were very high in the roots of 1- to 5-d-old seedlings, in which rapid root elongation takes place. Furthermore, GmEXP1 mRNA was most abundant in the root tip region, where cell elongation occurs, but scarce in the region of maturation, where cell elongation ceases, implying that its expression is closely related to root development processes. In situ hybridization showed that GmEXP1 transcripts were preferentially present in the epidermal cells and underlying cell layers in the root tip of the primary and secondary roots. Ectopic expression of GmEXP1 accelerated the root growth of transgenic tobacco (Nicotiana tabacum) seedlings, and the roots showed insensitivity to obstacle-touching stress. These results imply that the GmEXP1 gene plays an important role in root development in soybean, especially in the elongation and/or initiation of the primary and secondary roots.

Original languageEnglish
Pages (from-to)985-997
Number of pages13
JournalPlant Physiology
Volume131
Issue number3
DOIs
Publication statusPublished - 2003 Mar 1

Fingerprint

expansins
Soybeans
root growth
soybeans
Gene Expression
Meristem
Seedlings
genes
Tobacco
root tips
cell growth
Cell Wall
Genes
In Situ Hybridization
seedlings
Proteins
cells
Nicotiana tabacum
in situ hybridization
Messenger RNA

ASJC Scopus subject areas

  • Plant Science

Cite this

Expression of an expansin gene is correlated with root elongation in soybean. / Lee, Dong Keun; Ahn, Ji Hoon; Song, Sang Kee; Choi, Yang Do; Lee, Jong Seob.

In: Plant Physiology, Vol. 131, No. 3, 01.03.2003, p. 985-997.

Research output: Contribution to journalArticle

Lee, Dong Keun ; Ahn, Ji Hoon ; Song, Sang Kee ; Choi, Yang Do ; Lee, Jong Seob. / Expression of an expansin gene is correlated with root elongation in soybean. In: Plant Physiology. 2003 ; Vol. 131, No. 3. pp. 985-997.
@article{f914143f68344082b095a6768e4d9e6f,
title = "Expression of an expansin gene is correlated with root elongation in soybean",
abstract = "Expansin is a family of proteins that catalyze long-term expansion of cell walls and has been considered a principal protein that affects cell expansion in plants. We have identified the first root-specific expansin gene in soybean (Glycine max), GmEXP1, which may be responsible for root elongation. Expression levels of GmEXP1 were very high in the roots of 1- to 5-d-old seedlings, in which rapid root elongation takes place. Furthermore, GmEXP1 mRNA was most abundant in the root tip region, where cell elongation occurs, but scarce in the region of maturation, where cell elongation ceases, implying that its expression is closely related to root development processes. In situ hybridization showed that GmEXP1 transcripts were preferentially present in the epidermal cells and underlying cell layers in the root tip of the primary and secondary roots. Ectopic expression of GmEXP1 accelerated the root growth of transgenic tobacco (Nicotiana tabacum) seedlings, and the roots showed insensitivity to obstacle-touching stress. These results imply that the GmEXP1 gene plays an important role in root development in soybean, especially in the elongation and/or initiation of the primary and secondary roots.",
author = "Lee, {Dong Keun} and Ahn, {Ji Hoon} and Song, {Sang Kee} and Choi, {Yang Do} and Lee, {Jong Seob}",
year = "2003",
month = "3",
day = "1",
doi = "10.1104/pp.009902",
language = "English",
volume = "131",
pages = "985--997",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

TY - JOUR

T1 - Expression of an expansin gene is correlated with root elongation in soybean

AU - Lee, Dong Keun

AU - Ahn, Ji Hoon

AU - Song, Sang Kee

AU - Choi, Yang Do

AU - Lee, Jong Seob

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Expansin is a family of proteins that catalyze long-term expansion of cell walls and has been considered a principal protein that affects cell expansion in plants. We have identified the first root-specific expansin gene in soybean (Glycine max), GmEXP1, which may be responsible for root elongation. Expression levels of GmEXP1 were very high in the roots of 1- to 5-d-old seedlings, in which rapid root elongation takes place. Furthermore, GmEXP1 mRNA was most abundant in the root tip region, where cell elongation occurs, but scarce in the region of maturation, where cell elongation ceases, implying that its expression is closely related to root development processes. In situ hybridization showed that GmEXP1 transcripts were preferentially present in the epidermal cells and underlying cell layers in the root tip of the primary and secondary roots. Ectopic expression of GmEXP1 accelerated the root growth of transgenic tobacco (Nicotiana tabacum) seedlings, and the roots showed insensitivity to obstacle-touching stress. These results imply that the GmEXP1 gene plays an important role in root development in soybean, especially in the elongation and/or initiation of the primary and secondary roots.

AB - Expansin is a family of proteins that catalyze long-term expansion of cell walls and has been considered a principal protein that affects cell expansion in plants. We have identified the first root-specific expansin gene in soybean (Glycine max), GmEXP1, which may be responsible for root elongation. Expression levels of GmEXP1 were very high in the roots of 1- to 5-d-old seedlings, in which rapid root elongation takes place. Furthermore, GmEXP1 mRNA was most abundant in the root tip region, where cell elongation occurs, but scarce in the region of maturation, where cell elongation ceases, implying that its expression is closely related to root development processes. In situ hybridization showed that GmEXP1 transcripts were preferentially present in the epidermal cells and underlying cell layers in the root tip of the primary and secondary roots. Ectopic expression of GmEXP1 accelerated the root growth of transgenic tobacco (Nicotiana tabacum) seedlings, and the roots showed insensitivity to obstacle-touching stress. These results imply that the GmEXP1 gene plays an important role in root development in soybean, especially in the elongation and/or initiation of the primary and secondary roots.

UR - http://www.scopus.com/inward/record.url?scp=0037355068&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037355068&partnerID=8YFLogxK

U2 - 10.1104/pp.009902

DO - 10.1104/pp.009902

M3 - Article

VL - 131

SP - 985

EP - 997

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -