Expression of Corynebacterium glutamicum glycolytic genes varies with carbon source and growth phas

Sung Ok Han, Masayuki Inui, Hideaki Yukawa

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A basic pattern of gene expression and of relative expression levels during different growth phases was obtained for Corynebacterium glutamicum R grown on different carbon sources. The gapA-pgk-tpi-ppc gene cluster was transcribed as a mono- or polycistronic mRNA, depending on the growth phase. The 1.4 kb (gapA) and 2.3 kb (pgk-tip) mRNAs were expressed in the early through late exponential phases, whereas the 3.7 kb (gapA-pgk-tpi) and 5.4 kb (Pgk-tPi-PPc) mRNAs were only detected in the mid-exponential phase. All other glycolytic genes except pps, g1k and pgi were transcribed as monocistronic mRNAs under all tested conditions. Identification and alignment of the promoter regions of the transcriptional start sites of glycolytic genes revealed strong similarities to the σA consensus promoter sequences of Gram-positive bacteria. All genes involved in glycolysis were coordinately expressed in medium containing glucose. Growth in the presence of glucose gave rise to abundant expression of most glycolytic genes, with the level of gapA transcript being the highest. Glucose depletion led to a rapid repression of most glycolytic genes and a corresponding two- to fivefold increased expression of the gluconeogenic genes pps, pck and maIE, which are induced by pyruvate, lactate, acetate and/or other organic acids.

Original languageEnglish
Pages (from-to)2190-2202
Number of pages13
JournalMicrobiology
Volume153
Issue number7
DOIs
Publication statusPublished - 2007 Jul 1

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Corynebacterium glutamicum
Carbon
Growth
Messenger RNA
Genes
Glucose
Gene Expression
Consensus Sequence
Gram-Positive Bacteria
Glycolysis
Multigene Family
Pyruvic Acid
Genetic Promoter Regions
Lactic Acid
Acetates
Acids

ASJC Scopus subject areas

  • Microbiology

Cite this

Expression of Corynebacterium glutamicum glycolytic genes varies with carbon source and growth phas. / Han, Sung Ok; Inui, Masayuki; Yukawa, Hideaki.

In: Microbiology, Vol. 153, No. 7, 01.07.2007, p. 2190-2202.

Research output: Contribution to journalArticle

Han, Sung Ok ; Inui, Masayuki ; Yukawa, Hideaki. / Expression of Corynebacterium glutamicum glycolytic genes varies with carbon source and growth phas. In: Microbiology. 2007 ; Vol. 153, No. 7. pp. 2190-2202.
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