Expression of guanylin and uroguanylin mRNA in human nasal mucosa and nasal polyps

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6 Citations (Scopus)

Abstract

Objectives - Guanylin and uroguanylin are small, heat-stable peptides that were originally isolated from the small intestine and from urine, respectively. Functionally, it has been proposed that these peptides can regulate ion and water transport in various fluid-transporting epithelia. In the present study we evaluated the presence of mRNAs for human guanylin and uroguanylin in human inferior turbinate mucosa and nasal polyps. Material and Methods - The expression and localization of mRNAs for both peptides were investigated in inferior turbinate tissues and nasal polyps using reverse transcriptase polymerase chain reaction and in situ hybridization. Results - mRNAs for both peptides were detected in human turbinate mucosa and nasal polyps. In situ hybridization revealed that they were localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyps. However, their expression was noted at low levels in the superficial epithelium of nasal polyps, compared with that of inferior turbinate mucosa. Conclusions - These results indicate that guanylin and uroguanylin in the nasal mucosa may participate in normal physiological processes, but also suggest that altered expression of these genes in nasal polyps may cause impaired electrolyte and water transport across the epithelial cells.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalActa Oto-Laryngologica
Volume124
Issue number2
DOIs
Publication statusPublished - 2004 Mar 31

Fingerprint

Nasal Polyps
Nasal Mucosa
Turbinates
Messenger RNA
Mucous Membrane
Peptides
In Situ Hybridization
Epithelium
Physiological Phenomena
Water
Ion Transport
Reverse Transcriptase Polymerase Chain Reaction
Electrolytes
Small Intestine
guanylin
uroguanylin
Hot Temperature
Epithelial Cells
Urine
Gene Expression

Keywords

  • In situ hybridization
  • Inferior turbinate
  • Nasal polyps
  • Reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

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title = "Expression of guanylin and uroguanylin mRNA in human nasal mucosa and nasal polyps",
abstract = "Objectives - Guanylin and uroguanylin are small, heat-stable peptides that were originally isolated from the small intestine and from urine, respectively. Functionally, it has been proposed that these peptides can regulate ion and water transport in various fluid-transporting epithelia. In the present study we evaluated the presence of mRNAs for human guanylin and uroguanylin in human inferior turbinate mucosa and nasal polyps. Material and Methods - The expression and localization of mRNAs for both peptides were investigated in inferior turbinate tissues and nasal polyps using reverse transcriptase polymerase chain reaction and in situ hybridization. Results - mRNAs for both peptides were detected in human turbinate mucosa and nasal polyps. In situ hybridization revealed that they were localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyps. However, their expression was noted at low levels in the superficial epithelium of nasal polyps, compared with that of inferior turbinate mucosa. Conclusions - These results indicate that guanylin and uroguanylin in the nasal mucosa may participate in normal physiological processes, but also suggest that altered expression of these genes in nasal polyps may cause impaired electrolyte and water transport across the epithelial cells.",
keywords = "In situ hybridization, Inferior turbinate, Nasal polyps, Reverse transcriptase polymerase chain reaction",
author = "Lee, {Sang Hag} and Paeng, {Jae Pil} and Jung, {Hak Hyun} and Lee, {Seung Hoon} and Lee, {Heung Man} and Kwon, {Soon Young} and Lim, {Ki Jung} and Kwang-Yoon Jung",
year = "2004",
month = "3",
day = "31",
doi = "10.1080/00016480310016073",
language = "English",
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pages = "179--185",
journal = "Acta Oto-Laryngologica",
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TY - JOUR

T1 - Expression of guanylin and uroguanylin mRNA in human nasal mucosa and nasal polyps

AU - Lee, Sang Hag

AU - Paeng, Jae Pil

AU - Jung, Hak Hyun

AU - Lee, Seung Hoon

AU - Lee, Heung Man

AU - Kwon, Soon Young

AU - Lim, Ki Jung

AU - Jung, Kwang-Yoon

PY - 2004/3/31

Y1 - 2004/3/31

N2 - Objectives - Guanylin and uroguanylin are small, heat-stable peptides that were originally isolated from the small intestine and from urine, respectively. Functionally, it has been proposed that these peptides can regulate ion and water transport in various fluid-transporting epithelia. In the present study we evaluated the presence of mRNAs for human guanylin and uroguanylin in human inferior turbinate mucosa and nasal polyps. Material and Methods - The expression and localization of mRNAs for both peptides were investigated in inferior turbinate tissues and nasal polyps using reverse transcriptase polymerase chain reaction and in situ hybridization. Results - mRNAs for both peptides were detected in human turbinate mucosa and nasal polyps. In situ hybridization revealed that they were localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyps. However, their expression was noted at low levels in the superficial epithelium of nasal polyps, compared with that of inferior turbinate mucosa. Conclusions - These results indicate that guanylin and uroguanylin in the nasal mucosa may participate in normal physiological processes, but also suggest that altered expression of these genes in nasal polyps may cause impaired electrolyte and water transport across the epithelial cells.

AB - Objectives - Guanylin and uroguanylin are small, heat-stable peptides that were originally isolated from the small intestine and from urine, respectively. Functionally, it has been proposed that these peptides can regulate ion and water transport in various fluid-transporting epithelia. In the present study we evaluated the presence of mRNAs for human guanylin and uroguanylin in human inferior turbinate mucosa and nasal polyps. Material and Methods - The expression and localization of mRNAs for both peptides were investigated in inferior turbinate tissues and nasal polyps using reverse transcriptase polymerase chain reaction and in situ hybridization. Results - mRNAs for both peptides were detected in human turbinate mucosa and nasal polyps. In situ hybridization revealed that they were localized in the epithelial layer and submucosal glands of inferior turbinate mucosa and in the epithelial layer of nasal polyps. However, their expression was noted at low levels in the superficial epithelium of nasal polyps, compared with that of inferior turbinate mucosa. Conclusions - These results indicate that guanylin and uroguanylin in the nasal mucosa may participate in normal physiological processes, but also suggest that altered expression of these genes in nasal polyps may cause impaired electrolyte and water transport across the epithelial cells.

KW - In situ hybridization

KW - Inferior turbinate

KW - Nasal polyps

KW - Reverse transcriptase polymerase chain reaction

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