Expression of human β-defensin 1 mRNA in human nasal mucosa

Sang Hag Lee, Ho Lim Hyun, Heung Man Lee, Ouck Choi Jong

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Antimicrobial peptides are cationic proteins that are found in a wide range of organisms. Recent reports suggested that human β-defensin 1 (hBD- 1), a prominent group of antimicrobial peptides, is an important component of the innate immune response, particularly at mucosal surfaces that are vulnerable to colonization by potential pathogens. Therefore, hBD-1 may participate in providing intrinsic nasal mucosal defence against microbial infections. The present study aimed to look for hBD-1 mRNA in human nasal mucosa without obvious signs of inflammation. Total RNA was isolated from human inferior turbinate mucosa and hBD-1 mRNA was detected in these tissues by using reverse transcription and polymerase chain reaction (RT-PCR). By in situ hybridization, hBD-1 mRNA was predominantly localized in superficial epithelial cells and submucosal glandular epithelium of human inferior turbinate mucosa. These data suggest that nasal epithelia and submucosal glands may secrete hBD-1, contributing to the mucosal defences of the nose.

Original languageEnglish
Pages (from-to)58-61
Number of pages4
JournalActa Oto-Laryngologica
Volume120
Issue number1
Publication statusPublished - 2000 Jul 17

Fingerprint

Defensins
Nasal Mucosa
Turbinates
Messenger RNA
Nose
Mucous Membrane
Antimicrobial Cationic Peptides
Innate Immunity
Reverse Transcription
In Situ Hybridization
Epithelium
Epithelial Cells
RNA
Inflammation
Polymerase Chain Reaction
Peptides
Infection
Proteins

Keywords

  • In situ hybridization
  • Inferior turbinate mucosa
  • Mucosal defence
  • RT-PCR

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression of human β-defensin 1 mRNA in human nasal mucosa. / Lee, Sang Hag; Hyun, Ho Lim; Lee, Heung Man; Jong, Ouck Choi.

In: Acta Oto-Laryngologica, Vol. 120, No. 1, 17.07.2000, p. 58-61.

Research output: Contribution to journalArticle

Lee, Sang Hag ; Hyun, Ho Lim ; Lee, Heung Man ; Jong, Ouck Choi. / Expression of human β-defensin 1 mRNA in human nasal mucosa. In: Acta Oto-Laryngologica. 2000 ; Vol. 120, No. 1. pp. 58-61.
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