Expression of human interleukin-11 and granulocyte-macrophage colony-stimulating factor in transgenic plants

Bo Ye Lee, Jeong Hyun Lee, Hoon Seok Yoon, Kyung Ho Kang, Kyung Nam Kim, Jae-Hong Kim, Ju Kon Kim, Jeong Kook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The production of therapeutic proteins for human diseases in plants results in many economic benefits, including reduced risk of animal virus contamination, high yields, and reduced production and storage costs. Human cytokines, interleukin-11 (hIL-11) and granulocyte-macrophage colony-stimulating factor (hGM-CSF), cDNAs were introduced into rice or tobacco, using either the maize ubiquitin promoter or the 35S promoter. The primary hIL-11 transgenic rice plants exhibited stunted growth and a sterile phenotype, whereas the hIL-11 transgenic tobacco plants did not. This suggests that hIL-11 expression in rice disrupts the normal growth and development of the plant. The regeneration efficiency of rice calli transformed with hGM-CSF was found to be approximately a quarter of that seen with the hIL-11, suggesting that hGM-CSF expression is more deleterious to the regeneration of rice calli than is hIL-11. However, the surviving hGM-CSF transgenic rice plants exhibited a normal phenotype of growth. Therefore, it appears that only those transgenic rice lines that expressed the human cytokines in small quantities were able to survive the selection process.

Original languageEnglish
Pages (from-to)1304-1309
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number6
Publication statusPublished - 2005 Dec 1

Fingerprint

Interleukin-11
Macrophages
Genetically Modified Plants
Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Tobacco
Bony Callus
Regeneration
Growth Disorders
Phenotype
Plant Diseases
Ubiquitin
Viruses
Oryza
Growth and Development
Animals
Contamination
Zea mays
Complementary DNA
Proteins

Keywords

  • GM-CSF
  • Human cytokines
  • Interleukin-11
  • Rice
  • Tobacco
  • Transgenic plant

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Expression of human interleukin-11 and granulocyte-macrophage colony-stimulating factor in transgenic plants. / Lee, Bo Ye; Lee, Jeong Hyun; Yoon, Hoon Seok; Kang, Kyung Ho; Kim, Kyung Nam; Kim, Jae-Hong; Kim, Ju Kon; Kim, Jeong Kook.

In: Journal of Microbiology and Biotechnology, Vol. 15, No. 6, 01.12.2005, p. 1304-1309.

Research output: Contribution to journalArticle

Lee, Bo Ye ; Lee, Jeong Hyun ; Yoon, Hoon Seok ; Kang, Kyung Ho ; Kim, Kyung Nam ; Kim, Jae-Hong ; Kim, Ju Kon ; Kim, Jeong Kook. / Expression of human interleukin-11 and granulocyte-macrophage colony-stimulating factor in transgenic plants. In: Journal of Microbiology and Biotechnology. 2005 ; Vol. 15, No. 6. pp. 1304-1309.
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