Expression of human interleukin-11 and granulocyte-macrophage colony-stimulating factor in transgenic plants

Bo Ye Lee, Jeong Hyun Lee, Hoon Seok Yoon, Kyung Ho Kang, Kyung Nam Kim, Jae Hong Kim, Ju Kon Kim, Jeong Kook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The production of therapeutic proteins for human diseases in plants results in many economic benefits, including reduced risk of animal virus contamination, high yields, and reduced production and storage costs. Human cytokines, interleukin-11 (hIL-11) and granulocyte-macrophage colony-stimulating factor (hGM-CSF), cDNAs were introduced into rice or tobacco, using either the maize ubiquitin promoter or the 35S promoter. The primary hIL-11 transgenic rice plants exhibited stunted growth and a sterile phenotype, whereas the hIL-11 transgenic tobacco plants did not. This suggests that hIL-11 expression in rice disrupts the normal growth and development of the plant. The regeneration efficiency of rice calli transformed with hGM-CSF was found to be approximately a quarter of that seen with the hIL-11, suggesting that hGM-CSF expression is more deleterious to the regeneration of rice calli than is hIL-11. However, the surviving hGM-CSF transgenic rice plants exhibited a normal phenotype of growth. Therefore, it appears that only those transgenic rice lines that expressed the human cytokines in small quantities were able to survive the selection process.

Original languageEnglish
Pages (from-to)1304-1309
Number of pages6
JournalJournal of microbiology and biotechnology
Volume15
Issue number6
Publication statusPublished - 2005 Dec

Keywords

  • GM-CSF
  • Human cytokines
  • Interleukin-11
  • Rice
  • Tobacco
  • Transgenic plant

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Expression of human interleukin-11 and granulocyte-macrophage colony-stimulating factor in transgenic plants'. Together they form a unique fingerprint.

Cite this