Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Hee Won Lee; Jae Hoon Joo; Seongman Kang; In Sung Song; Jong Bum Kwon; Moon H. Han; Doe Sun Na

doi:10.1007/BF01021637

Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Hee Won Lee, Jae Hoon Joo, Seongman Kang, In Sung Song, Jong Bum Kwon, Moon H. Han, Doe Sun Na

Division of Life Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

Original language	English
Pages (from-to)	653-658
Number of pages	6
Journal	Biotechnology letters
Volume	14
Issue number	8
DOIs	https://doi.org/10.1007/BF01021637
Publication status	Published - 1992 Aug

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01021637

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Expression of human interleukin-2 from native and synthetic genes in E. coli : No correlation between major codon bias and high level expression. / Lee, Hee Won; Joo, Jae Hoon; Kang, Seongman; Song, In Sung; Kwon, Jong Bum; Han, Moon H.; Na, Doe Sun.

In: Biotechnology letters, Vol. 14, No. 8, 08.1992, p. 653-658.

Research output: Contribution to journal › Article › peer-review

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abstract = "Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.",

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AU - Lee, Hee Won

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AU - Kang, Seongman

AU - Song, In Sung

AU - Kwon, Jong Bum

AU - Han, Moon H.

AU - Na, Doe Sun

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AB - Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

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Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Abstract

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