Abstract
Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.
| Original language | English |
|---|---|
| Pages (from-to) | 653-658 |
| Number of pages | 6 |
| Journal | Biotechnology Letters |
| Volume | 14 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 1992 Aug 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
- Applied Microbiology and Biotechnology
- Bioengineering
- Biotechnology
Cite this
Expression of human interleukin-2 from native and synthetic genes in E. coli : No correlation between major codon bias and high level expression. / Lee, Hee Won; Joo, Jae Hoon; Kang, Seong Man; Song, In Sung; Kwon, Jong Bum; Han, Moon H.; Na, Doe Sun.
In: Biotechnology Letters, Vol. 14, No. 8, 01.08.1992, p. 653-658.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of human interleukin-2 from native and synthetic genes in E. coli
T2 - No correlation between major codon bias and high level expression
AU - Lee, Hee Won
AU - Joo, Jae Hoon
AU - Kang, Seong Man
AU - Song, In Sung
AU - Kwon, Jong Bum
AU - Han, Moon H.
AU - Na, Doe Sun
PY - 1992/8/1
Y1 - 1992/8/1
N2 - Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.
AB - Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.
UR - http://www.scopus.com/inward/record.url?scp=0026646014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026646014&partnerID=8YFLogxK
U2 - 10.1007/BF01021637
DO - 10.1007/BF01021637
M3 - Article
AN - SCOPUS:0026646014
VL - 14
SP - 653
EP - 658
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 8
ER -