Expression of human interleukin-2 from native and synthetic genes in E. coli: No correlation between major codon bias and high level expression

Hee Won Lee, Jae Hoon Joo, Seong Man Kang, In Sung Song, Jong Bum Kwon, Moon H. Han, Doe Sun Na

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Expression of human IL-2 from the cDNA and synthetic gene was compared using the E. coli expression system. Expression plasmids containing the cDNA or synthetic gene in which the only difference was the coding sequences were constructed. Expression of IL-2 from the cDNA and synthetic gene were estimated to be as high as 18% and 14%, respectively. No correlation was found between the major codon bias and high level expression of IL-2 in this system.

Original languageEnglish
Pages (from-to)653-658
Number of pages6
JournalBiotechnology Letters
Volume14
Issue number8
DOIs
Publication statusPublished - 1992 Aug 1
Externally publishedYes

Fingerprint

Synthetic Genes
Codon
Escherichia coli
Interleukin-2
Complementary DNA
Genes
Plasmids

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

Cite this

Expression of human interleukin-2 from native and synthetic genes in E. coli : No correlation between major codon bias and high level expression. / Lee, Hee Won; Joo, Jae Hoon; Kang, Seong Man; Song, In Sung; Kwon, Jong Bum; Han, Moon H.; Na, Doe Sun.

In: Biotechnology Letters, Vol. 14, No. 8, 01.08.1992, p. 653-658.

Research output: Contribution to journalArticle

Lee, Hee Won ; Joo, Jae Hoon ; Kang, Seong Man ; Song, In Sung ; Kwon, Jong Bum ; Han, Moon H. ; Na, Doe Sun. / Expression of human interleukin-2 from native and synthetic genes in E. coli : No correlation between major codon bias and high level expression. In: Biotechnology Letters. 1992 ; Vol. 14, No. 8. pp. 653-658.
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