Expression of insulin-like growth factors in a mouse model of salicylate ototoxicity

Gi Jung Im, June Choi, Ji Won Chang, Seo Jin Kim, Hye In Kim, Hak Hyun Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives. Insulin-like growth factors (IGFs) are known to be neurotrophic factors, and they efficiently signal to cells to grow, differentiate and survive. The purpose of study was to identify the expressions of IGFs in mice with salicylate ototoxicity, which is a typical reversible hearing loss model. Methods. The mice were given intraperitoneal injections of salicylate (400 mg/kg) and about a 30 dB threshold shift was achieved at 3 hours. The expressions of IGF-1 and 2 were confirmed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Localization of IGFs was confirmed using confocal immunofluorescence imaging. For in-vitro study on the HEI-OC1 auditory cells, the cell viability was calculated and the apoptotic features of the nuclei were observed with Hoechst staining. Results. The expressions of the IGFs mRNA and protein were significantly increased in the salicylate ototoxicity groups compared with that of the normal control group. Salicylate induced apoptosis and decreased viability of the HEI-OC1 auditory cells in a time- and dose-dependent manner. The expressions of IGFs were localized in the stria vascularis, and these IGFs play a protective role in the in-vivo condition of salicylate ototoxicity. Conclusion. IGFs were highly expressed in the mice with salicylate ototoxicity, and this expression was mainly focused in the stria vascularis in the salicylate intoxicated mice. The systemic action of IGFs, which were expressed in the vascular-rich stria vascularis, can act as a major protective mechanism in a mouse model of salicylate ototoxicity.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalClinical and Experimental Otorhinolaryngology
Volume3
Issue number3
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Salicylates
Somatomedins
Stria Vascularis
Nerve Growth Factors
Intraperitoneal Injections
Hearing Loss
Reverse Transcription
Fluorescent Antibody Technique
Blood Vessels
Cell Survival
Western Blotting
Apoptosis
Staining and Labeling
Polymerase Chain Reaction
Control Groups
Messenger RNA

Keywords

  • Cochlea
  • Insulin-like growth factor
  • Ototoxicity
  • Salicylate

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Expression of insulin-like growth factors in a mouse model of salicylate ototoxicity. / Im, Gi Jung; Choi, June; Chang, Ji Won; Kim, Seo Jin; Kim, Hye In; Jung, Hak Hyun.

In: Clinical and Experimental Otorhinolaryngology, Vol. 3, No. 3, 01.09.2010, p. 115-121.

Research output: Contribution to journalArticle

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