Expression of myosin heavy chain mRNA in rat laryngeal muscles

Hak Hyun Jung, Seung Hoon Han, Jong Ouck Choi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The composition of myosin heavy chain mRNA was analysed quantitatively in 5 intrinsic laryngeal muscles of rats, using a competitive polymerase chain reaction. Intrinsic laryngeal muscles with the fastest contraction times, e.g. ventricular thyroarytenoid muscle, lateral cricoarytenoid muscle, and vocalis muscle, contained 2 fast isoforms, comprising mainly type 2B myosin heavy chains (52.1, 44.6 and 8.2%, respectively) and type 2X myosin heavy chains (21.9, 37.6 and 80.8%, respectively). Conversely, muscles with slower contraction times, such as posterior cricoarytenoid muscle and cricothyroid muscle, contained more than 85% of 2 fast isoforms; mainly type 2X myosin heavy chains (52.4-72.1%, respectively) and type 2A myosin heavy chains (34.6-25.2%, respectively). The results show a strong correlation between the composition of fast myosin heavy chain isoforms and muscle contraction times. Type 2L myosin heavy chain transcripts specific for laryngeal muscles and extra-ocular muscles were expressed in the order of ventricular thyroarytenoid (9.5%) > lateral cricoarytenoid (4.8%) > vocalis (2.5%) > posterior cricoarytenoid muscle (0.9%), but were not expressed in cricothyroid muscle. Neonatal myosin heavy chain was also expressed in all laryngeal muscles, ranging from 0.04 to 3%, but embryonic myosin heavy chain was expressed in ventricular thyroarytenoid, posterior cricoarytenoid and cricothyroid muscle at very low levels. These results suggest that intrinsic laryngeal muscles have different expression patterns for myosin heavy chain isoforms and may have different regulatory roles related to their functional requirement.

Original languageEnglish
Pages (from-to)396-402
Number of pages7
JournalActa Oto-Laryngologica
Volume119
Issue number3
DOIs
Publication statusPublished - 1999 May 25

Fingerprint

Laryngeal Muscles
Myosin Heavy Chains
Messenger RNA
Protein Isoforms
Muscles
Muscle Contraction

Keywords

  • Gene expression
  • Polymerase chain reaction
  • Rat

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression of myosin heavy chain mRNA in rat laryngeal muscles. / Jung, Hak Hyun; Han, Seung Hoon; Choi, Jong Ouck.

In: Acta Oto-Laryngologica, Vol. 119, No. 3, 25.05.1999, p. 396-402.

Research output: Contribution to journalArticle

Jung, Hak Hyun ; Han, Seung Hoon ; Choi, Jong Ouck. / Expression of myosin heavy chain mRNA in rat laryngeal muscles. In: Acta Oto-Laryngologica. 1999 ; Vol. 119, No. 3. pp. 396-402.
@article{8e0b75ebf2964a1c81bb42565fa89bed,
title = "Expression of myosin heavy chain mRNA in rat laryngeal muscles",
abstract = "The composition of myosin heavy chain mRNA was analysed quantitatively in 5 intrinsic laryngeal muscles of rats, using a competitive polymerase chain reaction. Intrinsic laryngeal muscles with the fastest contraction times, e.g. ventricular thyroarytenoid muscle, lateral cricoarytenoid muscle, and vocalis muscle, contained 2 fast isoforms, comprising mainly type 2B myosin heavy chains (52.1, 44.6 and 8.2{\%}, respectively) and type 2X myosin heavy chains (21.9, 37.6 and 80.8{\%}, respectively). Conversely, muscles with slower contraction times, such as posterior cricoarytenoid muscle and cricothyroid muscle, contained more than 85{\%} of 2 fast isoforms; mainly type 2X myosin heavy chains (52.4-72.1{\%}, respectively) and type 2A myosin heavy chains (34.6-25.2{\%}, respectively). The results show a strong correlation between the composition of fast myosin heavy chain isoforms and muscle contraction times. Type 2L myosin heavy chain transcripts specific for laryngeal muscles and extra-ocular muscles were expressed in the order of ventricular thyroarytenoid (9.5{\%}) > lateral cricoarytenoid (4.8{\%}) > vocalis (2.5{\%}) > posterior cricoarytenoid muscle (0.9{\%}), but were not expressed in cricothyroid muscle. Neonatal myosin heavy chain was also expressed in all laryngeal muscles, ranging from 0.04 to 3{\%}, but embryonic myosin heavy chain was expressed in ventricular thyroarytenoid, posterior cricoarytenoid and cricothyroid muscle at very low levels. These results suggest that intrinsic laryngeal muscles have different expression patterns for myosin heavy chain isoforms and may have different regulatory roles related to their functional requirement.",
keywords = "Gene expression, Polymerase chain reaction, Rat",
author = "Jung, {Hak Hyun} and Han, {Seung Hoon} and Choi, {Jong Ouck}",
year = "1999",
month = "5",
day = "25",
doi = "10.1080/00016489950181459",
language = "English",
volume = "119",
pages = "396--402",
journal = "Acta Oto-Laryngologica",
issn = "0001-6489",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Expression of myosin heavy chain mRNA in rat laryngeal muscles

AU - Jung, Hak Hyun

AU - Han, Seung Hoon

AU - Choi, Jong Ouck

PY - 1999/5/25

Y1 - 1999/5/25

N2 - The composition of myosin heavy chain mRNA was analysed quantitatively in 5 intrinsic laryngeal muscles of rats, using a competitive polymerase chain reaction. Intrinsic laryngeal muscles with the fastest contraction times, e.g. ventricular thyroarytenoid muscle, lateral cricoarytenoid muscle, and vocalis muscle, contained 2 fast isoforms, comprising mainly type 2B myosin heavy chains (52.1, 44.6 and 8.2%, respectively) and type 2X myosin heavy chains (21.9, 37.6 and 80.8%, respectively). Conversely, muscles with slower contraction times, such as posterior cricoarytenoid muscle and cricothyroid muscle, contained more than 85% of 2 fast isoforms; mainly type 2X myosin heavy chains (52.4-72.1%, respectively) and type 2A myosin heavy chains (34.6-25.2%, respectively). The results show a strong correlation between the composition of fast myosin heavy chain isoforms and muscle contraction times. Type 2L myosin heavy chain transcripts specific for laryngeal muscles and extra-ocular muscles were expressed in the order of ventricular thyroarytenoid (9.5%) > lateral cricoarytenoid (4.8%) > vocalis (2.5%) > posterior cricoarytenoid muscle (0.9%), but were not expressed in cricothyroid muscle. Neonatal myosin heavy chain was also expressed in all laryngeal muscles, ranging from 0.04 to 3%, but embryonic myosin heavy chain was expressed in ventricular thyroarytenoid, posterior cricoarytenoid and cricothyroid muscle at very low levels. These results suggest that intrinsic laryngeal muscles have different expression patterns for myosin heavy chain isoforms and may have different regulatory roles related to their functional requirement.

AB - The composition of myosin heavy chain mRNA was analysed quantitatively in 5 intrinsic laryngeal muscles of rats, using a competitive polymerase chain reaction. Intrinsic laryngeal muscles with the fastest contraction times, e.g. ventricular thyroarytenoid muscle, lateral cricoarytenoid muscle, and vocalis muscle, contained 2 fast isoforms, comprising mainly type 2B myosin heavy chains (52.1, 44.6 and 8.2%, respectively) and type 2X myosin heavy chains (21.9, 37.6 and 80.8%, respectively). Conversely, muscles with slower contraction times, such as posterior cricoarytenoid muscle and cricothyroid muscle, contained more than 85% of 2 fast isoforms; mainly type 2X myosin heavy chains (52.4-72.1%, respectively) and type 2A myosin heavy chains (34.6-25.2%, respectively). The results show a strong correlation between the composition of fast myosin heavy chain isoforms and muscle contraction times. Type 2L myosin heavy chain transcripts specific for laryngeal muscles and extra-ocular muscles were expressed in the order of ventricular thyroarytenoid (9.5%) > lateral cricoarytenoid (4.8%) > vocalis (2.5%) > posterior cricoarytenoid muscle (0.9%), but were not expressed in cricothyroid muscle. Neonatal myosin heavy chain was also expressed in all laryngeal muscles, ranging from 0.04 to 3%, but embryonic myosin heavy chain was expressed in ventricular thyroarytenoid, posterior cricoarytenoid and cricothyroid muscle at very low levels. These results suggest that intrinsic laryngeal muscles have different expression patterns for myosin heavy chain isoforms and may have different regulatory roles related to their functional requirement.

KW - Gene expression

KW - Polymerase chain reaction

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0032933195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032933195&partnerID=8YFLogxK

U2 - 10.1080/00016489950181459

DO - 10.1080/00016489950181459

M3 - Article

VL - 119

SP - 396

EP - 402

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

IS - 3

ER -