Expression of neutrophil gelatinase-associated lipocalin in nasal polyps

Hyun Jae Woo, Jeong Ki Min, Chang Hoon Bai, Si Youn Song, Hee Joon Kang, Heung Man Lee, Yong Dae Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To investigate the expression and localization of neutrophil gelatinase-associated lipocalin (NGAL), an antimicrobial peptide, in the normal nasal mucosa and human nasal polyps. Neutrophil gelatinase-associated lipocalin has been identified as a key element in the innate host defense system. However, scant knowledge exists about the expression of NGAL in the human sinonasal tract. Design: Prospective study. Setting: Academic medical center. Patients: Normal inferior turbinate mucosa was obtained from 10 patients who were undergoing augmentation rhinoplasty. The nasal polyps were obtained from 10 patients who were undergoing endoscopic sinus surgery for chronic rhinosinusitis with polyps. Interventions: We performed semiquantitative reverse transcription-polymerase chain reaction, immunohistochemical staining, and Western blot analysis. Main Outcome Measures: We analyzed the expression of the NGAL messenger RNA (mRNA) and localization of the NGAL protein. Results: The NGAL mRNA and NGAL protein were highly expressed in the nasal polyps. The ratio of NGAL mRNA to glyceraldehyde-3-phosphate dehydrogenase mRNA in the nasal polyps was greater compared with that in the normal turbinate mucosa (P=.002). The NGAL protein was observed in the epithelium, the infiltrating inflammatory cells, and the submucosal gland of the nasal polyps, but it was very rarely detected in the normal nasal mucosa. Conclusion: Expression of NGAL is upregulated in nasal polyps, and additional work is needed to reveal the possible role of NGAL in the defense systems of the nasal mucosa and the process of polyp formation.

Original languageEnglish
Pages (from-to)1182-1186
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume134
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

Fingerprint

Nasal Polyps
Nasal Mucosa
Turbinates
Messenger RNA
Polyps
Mucous Membrane
Lipocalin-2
Rhinoplasty
Glyceraldehyde-3-Phosphate Dehydrogenases
Proteins
Reverse Transcription
Epithelium
Western Blotting
Outcome Assessment (Health Care)

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Expression of neutrophil gelatinase-associated lipocalin in nasal polyps. / Woo, Hyun Jae; Min, Jeong Ki; Bai, Chang Hoon; Song, Si Youn; Kang, Hee Joon; Lee, Heung Man; Kim, Yong Dae.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 134, No. 11, 01.11.2008, p. 1182-1186.

Research output: Contribution to journalArticle

Woo, Hyun Jae ; Min, Jeong Ki ; Bai, Chang Hoon ; Song, Si Youn ; Kang, Hee Joon ; Lee, Heung Man ; Kim, Yong Dae. / Expression of neutrophil gelatinase-associated lipocalin in nasal polyps. In: Archives of Otolaryngology - Head and Neck Surgery. 2008 ; Vol. 134, No. 11. pp. 1182-1186.
@article{bea9fe5360b74d1e9586dd996b9c655a,
title = "Expression of neutrophil gelatinase-associated lipocalin in nasal polyps",
abstract = "Objective: To investigate the expression and localization of neutrophil gelatinase-associated lipocalin (NGAL), an antimicrobial peptide, in the normal nasal mucosa and human nasal polyps. Neutrophil gelatinase-associated lipocalin has been identified as a key element in the innate host defense system. However, scant knowledge exists about the expression of NGAL in the human sinonasal tract. Design: Prospective study. Setting: Academic medical center. Patients: Normal inferior turbinate mucosa was obtained from 10 patients who were undergoing augmentation rhinoplasty. The nasal polyps were obtained from 10 patients who were undergoing endoscopic sinus surgery for chronic rhinosinusitis with polyps. Interventions: We performed semiquantitative reverse transcription-polymerase chain reaction, immunohistochemical staining, and Western blot analysis. Main Outcome Measures: We analyzed the expression of the NGAL messenger RNA (mRNA) and localization of the NGAL protein. Results: The NGAL mRNA and NGAL protein were highly expressed in the nasal polyps. The ratio of NGAL mRNA to glyceraldehyde-3-phosphate dehydrogenase mRNA in the nasal polyps was greater compared with that in the normal turbinate mucosa (P=.002). The NGAL protein was observed in the epithelium, the infiltrating inflammatory cells, and the submucosal gland of the nasal polyps, but it was very rarely detected in the normal nasal mucosa. Conclusion: Expression of NGAL is upregulated in nasal polyps, and additional work is needed to reveal the possible role of NGAL in the defense systems of the nasal mucosa and the process of polyp formation.",
author = "Woo, {Hyun Jae} and Min, {Jeong Ki} and Bai, {Chang Hoon} and Song, {Si Youn} and Kang, {Hee Joon} and Lee, {Heung Man} and Kim, {Yong Dae}",
year = "2008",
month = "11",
day = "1",
doi = "10.1001/archotol.134.11.1182",
language = "English",
volume = "134",
pages = "1182--1186",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "11",

}

TY - JOUR

T1 - Expression of neutrophil gelatinase-associated lipocalin in nasal polyps

AU - Woo, Hyun Jae

AU - Min, Jeong Ki

AU - Bai, Chang Hoon

AU - Song, Si Youn

AU - Kang, Hee Joon

AU - Lee, Heung Man

AU - Kim, Yong Dae

PY - 2008/11/1

Y1 - 2008/11/1

N2 - Objective: To investigate the expression and localization of neutrophil gelatinase-associated lipocalin (NGAL), an antimicrobial peptide, in the normal nasal mucosa and human nasal polyps. Neutrophil gelatinase-associated lipocalin has been identified as a key element in the innate host defense system. However, scant knowledge exists about the expression of NGAL in the human sinonasal tract. Design: Prospective study. Setting: Academic medical center. Patients: Normal inferior turbinate mucosa was obtained from 10 patients who were undergoing augmentation rhinoplasty. The nasal polyps were obtained from 10 patients who were undergoing endoscopic sinus surgery for chronic rhinosinusitis with polyps. Interventions: We performed semiquantitative reverse transcription-polymerase chain reaction, immunohistochemical staining, and Western blot analysis. Main Outcome Measures: We analyzed the expression of the NGAL messenger RNA (mRNA) and localization of the NGAL protein. Results: The NGAL mRNA and NGAL protein were highly expressed in the nasal polyps. The ratio of NGAL mRNA to glyceraldehyde-3-phosphate dehydrogenase mRNA in the nasal polyps was greater compared with that in the normal turbinate mucosa (P=.002). The NGAL protein was observed in the epithelium, the infiltrating inflammatory cells, and the submucosal gland of the nasal polyps, but it was very rarely detected in the normal nasal mucosa. Conclusion: Expression of NGAL is upregulated in nasal polyps, and additional work is needed to reveal the possible role of NGAL in the defense systems of the nasal mucosa and the process of polyp formation.

AB - Objective: To investigate the expression and localization of neutrophil gelatinase-associated lipocalin (NGAL), an antimicrobial peptide, in the normal nasal mucosa and human nasal polyps. Neutrophil gelatinase-associated lipocalin has been identified as a key element in the innate host defense system. However, scant knowledge exists about the expression of NGAL in the human sinonasal tract. Design: Prospective study. Setting: Academic medical center. Patients: Normal inferior turbinate mucosa was obtained from 10 patients who were undergoing augmentation rhinoplasty. The nasal polyps were obtained from 10 patients who were undergoing endoscopic sinus surgery for chronic rhinosinusitis with polyps. Interventions: We performed semiquantitative reverse transcription-polymerase chain reaction, immunohistochemical staining, and Western blot analysis. Main Outcome Measures: We analyzed the expression of the NGAL messenger RNA (mRNA) and localization of the NGAL protein. Results: The NGAL mRNA and NGAL protein were highly expressed in the nasal polyps. The ratio of NGAL mRNA to glyceraldehyde-3-phosphate dehydrogenase mRNA in the nasal polyps was greater compared with that in the normal turbinate mucosa (P=.002). The NGAL protein was observed in the epithelium, the infiltrating inflammatory cells, and the submucosal gland of the nasal polyps, but it was very rarely detected in the normal nasal mucosa. Conclusion: Expression of NGAL is upregulated in nasal polyps, and additional work is needed to reveal the possible role of NGAL in the defense systems of the nasal mucosa and the process of polyp formation.

UR - http://www.scopus.com/inward/record.url?scp=56649092867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56649092867&partnerID=8YFLogxK

U2 - 10.1001/archotol.134.11.1182

DO - 10.1001/archotol.134.11.1182

M3 - Article

VL - 134

SP - 1182

EP - 1186

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 11

ER -