Expression of secretory leukocyte protease inhibitor in middle ear cholesteatoma

Jung K. Lee, Sungwon Chae, Jae-Gu Cho, Heung Man Lee, Soon J. Hwang, Hak Hyun Jung

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Secretory leukocyte protease inhibitor (SLPI) is a cationic protein and a member of the innate immunity-associated protein family. The main function of SLPI is to protect local tissue against the detrimental consequences of inflammation. We undertook this study to investigate the expression of SLPI in human middle ear cholesteatoma tissue as compared with normal external auditory canal skin. Cholesteatoma tissues and external auditory canal skin samples were obtained from eight patients during middle ear surgery. The expression levels of SLPI mRNA and protein were evaluated by real-time reverse transcription- polymerase chain reaction (RT-PCR) and western blotting. Immunohistochemical staining was performed in both tissue groups using anti-SLPI antibody. Real-time RT-PCR showed that SLPI mRNA levels in cholesteatoma tissues were increased 7.8-fold on average as compared with normal auditory canal skin. Western blotting analysis showed that SLPI protein expression in cholesteatoma epithelium is up-regulated versus external auditory canal skin epithelium. Immunohistochemical staining for SLPI showed that SLPI is expressed mainly in the stratum granulosum and in subepithelial inflammatory cells. These findings imply that SLPI contributes to host protection against inflammatory cell and destructive enzymes in the chronic inflammatory state of cholesteatoma by affecting the innate immune system.

Original languageEnglish
Pages (from-to)1077-1081
Number of pages5
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume263
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

Secretory Leukocyte Peptidase Inhibitor
Middle Ear Cholesteatoma
Cholesteatoma
Ear Canal
Skin
Reverse Transcription
Proteins
Epithelium
Western Blotting
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
Middle Ear
Innate Immunity
Immune System

Keywords

  • Middle ear cholesteatoma
  • Secretory leukocyte protease inhibitor

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Expression of secretory leukocyte protease inhibitor in middle ear cholesteatoma. / Lee, Jung K.; Chae, Sungwon; Cho, Jae-Gu; Lee, Heung Man; Hwang, Soon J.; Jung, Hak Hyun.

In: European Archives of Oto-Rhino-Laryngology, Vol. 263, No. 12, 01.12.2006, p. 1077-1081.

Research output: Contribution to journalArticle

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