Expression of SOCS1 and SOCS3 is altered in the nasal mucosa of patients with mild and moderate/severe persistent allergic rhinitis

Sang Hag Lee, Tae-Hoon Kim, Kihyoung Kim, Se Jin Park, Sang Hag Lee, Jae Woong Hwang, Sang Heon Park, Gun Hwee Yum

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: The aim of this study was to evaluate the role of suppressor of cytokine signaling (SOCS) molecules, SOCS1 and SOCS3, which act as negative regulators of cytokine signaling in various allergic diseases, in patients with mild and moderate/severe persistent allergic rhinitis. Methods: The expression and distribution pattern of SOCS1 and SOCS3 were analyzed in nasal mucosa and peripheral blood mononuclear cells (PBMC) of healthy controls, and patients with mild and moderate/severe persistent allergic rhinitis using RT-PCR, immunohistochemistry and Western blotting. IL-4, IL-13, IL-15 and IFN-γ expression was also analyzed in the nasal mucosa of each individual using RT-PCR and Western blotting. Results: SOCS1 and SOCS3 mRNA and protein expression was significantly increased in the nasal mucosa and PBMC of patients with mild and moderate/severe persistent allergic rhinitis compared with healthy controls. In healthy and allergic nasal mucosa, they were commonly localized to the epithelium, submucosal glands and endothelium, showing stronger staining intensity in mild and moderate/severe persistent allergic nasal mucosa than in healthy nasal mucosa. Tissue levels of IL-4 and IL-13 were increased in moderate/severe persistent allergic nasal mucosa whereas IL-15 and IFN-γ were decreased in moderate/severe persistent allergic nasal mucosa. Conclusions: Upregulation of SOCS1 and SOCS3 in mild and moderate/severe persistent allergic rhinitis suggests that SOCS proteins may be important regulators in the pathogenesis of allergic rhinitis and play a role as molecular determinants of allergic rhinitis persistence.

Original languageEnglish
Pages (from-to)387-396
Number of pages10
JournalInternational Archives of Allergy and Immunology
Volume158
Issue number4
DOIs
Publication statusPublished - 2012 Jun 1

Fingerprint

Nasal Mucosa
Interleukin-15
Interleukin-13
Interleukin-4
Blood Cells
Suppressor of Cytokine Signaling Proteins
Western Blotting
Cytokines
Polymerase Chain Reaction
Allergic Rhinitis
Endothelium
Up-Regulation
Epithelium
Immunohistochemistry
Staining and Labeling
Messenger RNA

Keywords

  • Allergic nasal mucosa
  • Healthy nasal mucosa
  • Persistent allergy
  • Rhinitis
  • SOCS1
  • SOCS3

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Expression of SOCS1 and SOCS3 is altered in the nasal mucosa of patients with mild and moderate/severe persistent allergic rhinitis. / Lee, Sang Hag; Kim, Tae-Hoon; Kim, Kihyoung; Park, Se Jin; Lee, Sang Hag; Hwang, Jae Woong; Park, Sang Heon; Yum, Gun Hwee.

In: International Archives of Allergy and Immunology, Vol. 158, No. 4, 01.06.2012, p. 387-396.

Research output: Contribution to journalArticle

Lee, Sang Hag ; Kim, Tae-Hoon ; Kim, Kihyoung ; Park, Se Jin ; Lee, Sang Hag ; Hwang, Jae Woong ; Park, Sang Heon ; Yum, Gun Hwee. / Expression of SOCS1 and SOCS3 is altered in the nasal mucosa of patients with mild and moderate/severe persistent allergic rhinitis. In: International Archives of Allergy and Immunology. 2012 ; Vol. 158, No. 4. pp. 387-396.
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