Expression of the mexA gene requires the DNA helicase RecG in Pseudomonas aeruginosa PAO1

Aram Heo, Woojun Park

Research output: Contribution to journalArticle

Abstract

This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.

Original languageEnglish
Pages (from-to)492-495
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume25
Issue number4
DOIs
Publication statusPublished - 2015

Fingerprint

DNA Helicases
Pseudomonas aeruginosa
Gene Expression
Northern Blotting
Genes
Real-Time Polymerase Chain Reaction
Carrier Proteins

Keywords

  • DNA helicase RecG
  • Efflux pump
  • mexA
  • OxyR
  • Pseudomonas aerruginosa
  • Transcriptional regulator

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Expression of the mexA gene requires the DNA helicase RecG in Pseudomonas aeruginosa PAO1. / Heo, Aram; Park, Woojun.

In: Journal of Microbiology and Biotechnology, Vol. 25, No. 4, 2015, p. 492-495.

Research output: Contribution to journalArticle

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