Expression of thermosensitive two-pore domain K+ channels in human keratinocytes cell line HaCaT cells

Dawon Kang, Sung Hee Kim, Eun Mi Hwang, Oh Sang Kwon, Hae Young Yang, Eun Sook Kim, Tae Hyun Choi, Jae Yong Park, Seong Geun Hong, Jaehee Han

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Recent studies have shown that keratinocytes can sense temperature via thermo-transient receptor potential (TRP) channels. It is not known whether other thermosensitive ion channels such as TREK-1, TREK-2 and TRAAK (TREKs/TRAAK) that are members of the two-pore domain K+ (K2P) channel family are expressed in human keratinocytes. Here, we identified the expression of TREKs/TRAAK in human keratinocytes-derived cell line HaCaT cells using RT-PCR, immunocytochemistry, Western blot analysis and patch-clamp technique. RT-PCR showed that all six K2P channels tested (TASK-1, TASK-3, TREK-1, TREK-2, TRAAK and TASK-2) were expressed in HaCaT cells, as well as in skin and dorsal root ganglion (DRG) of rat. The expression of TREKs/TRAAK mRNA identified by RT-PCR was further studied at the protein level. Using anti-TREK-1, -TREK-2 and -TRAAK, bands of ∼46, ∼60 and ∼43 kDa, respectively, were observed at plasma membrane of HaCaT cells. Immunostaining also showed that TREK-1, TREK-2 and TRAAK were expressed in all area of cells including plasma membrane. Whole-cell K+ currents recorded from HaCaT cells were activated by arachidonic acid and heat. These results suggest that TREKs/TRAAK channels could act as thermosensors in human keratinocytes.

Original languageEnglish
Pages (from-to)1016-1022
Number of pages7
JournalExperimental Dermatology
Volume16
Issue number12
DOIs
Publication statusPublished - 2007 Dec
Externally publishedYes

Keywords

  • Keratinocytes
  • Potassium channels
  • Tandem-pore domain
  • Temperature sense

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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