Expression of thymosin β in the rat brain following transient global ischemia

Younghwa Kim, Eun Hae Kim, Soontaek Hong, Im Joo Rhyu, Jeehyung Choe, Woong Sun, Hyun Kim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thymosin β (Tβ) isoforms play an important role in the organization of the cytoskeleton by sequestering G-actin during development of the mammalian brain. In this study, we examined changes in the expression of Tβ4 and Tβ15 after transient global ischemia. Tβ15 mRNA increased gradually in the dentate gyrus (DG) of the hippocampal formation from 3 h after reperfusion and peaked 9 h later. Similarly, a significant increase in Tβ4 mRNA level was observed in the DG 12 h after reperfusion. Tβ4 and Tβ15 proteins were found in different cell types in control brains; Tβ15 was expressed in a subset of doublecortin (DCX)-positive cells in the DG, whereas Tβ4-IR was observed in DG neurons and nearby microglial cells. After ischemia, Tβ15-IR was found in DG neurons and Tβ4-IR in the reactivated microglial cells. Interestingly, Tβ15-IR accumulated in the nuclei of CA1 neurons, which are vulnerable to ischemic insults. These results suggest that Tβ4 and Tβ15 function in different cellular contexts during ischemia-induced responses.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalBrain Research
Volume1085
Issue number1
DOIs
Publication statusPublished - 2006 Apr 26
Externally publishedYes

Keywords

  • Actin cytoskeleton
  • Ischemia
  • Nuclear accumulation
  • Thymosin beta

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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