Expression of thymosin β in the rat brain following transient global ischemia

Younghwa Kim, Eun Hae Kim, Soontaek Hong, Im Joo Rhyu, Jeehyung Choe, Woong Sun, Hyun Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Thymosin β (Tβ) isoforms play an important role in the organization of the cytoskeleton by sequestering G-actin during development of the mammalian brain. In this study, we examined changes in the expression of Tβ4 and Tβ15 after transient global ischemia. Tβ15 mRNA increased gradually in the dentate gyrus (DG) of the hippocampal formation from 3 h after reperfusion and peaked 9 h later. Similarly, a significant increase in Tβ4 mRNA level was observed in the DG 12 h after reperfusion. Tβ4 and Tβ15 proteins were found in different cell types in control brains; Tβ15 was expressed in a subset of doublecortin (DCX)-positive cells in the DG, whereas Tβ4-IR was observed in DG neurons and nearby microglial cells. After ischemia, Tβ15-IR was found in DG neurons and Tβ4-IR in the reactivated microglial cells. Interestingly, Tβ15-IR accumulated in the nuclei of CA1 neurons, which are vulnerable to ischemic insults. These results suggest that Tβ4 and Tβ15 function in different cellular contexts during ischemia-induced responses.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalBrain Research
Volume1085
Issue number1
DOIs
Publication statusPublished - 2006 Apr 26
Externally publishedYes

Fingerprint

Thymosin
Dentate Gyrus
Ischemia
Brain
Neurons
Reperfusion
Messenger RNA
Cytoskeleton
Actins
Hippocampus
Protein Isoforms
Proteins

Keywords

  • Actin cytoskeleton
  • Ischemia
  • Nuclear accumulation
  • Thymosin beta

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Expression of thymosin β in the rat brain following transient global ischemia. / Kim, Younghwa; Kim, Eun Hae; Hong, Soontaek; Rhyu, Im Joo; Choe, Jeehyung; Sun, Woong; Kim, Hyun.

In: Brain Research, Vol. 1085, No. 1, 26.04.2006, p. 177-182.

Research output: Contribution to journalArticle

Kim, Younghwa ; Kim, Eun Hae ; Hong, Soontaek ; Rhyu, Im Joo ; Choe, Jeehyung ; Sun, Woong ; Kim, Hyun. / Expression of thymosin β in the rat brain following transient global ischemia. In: Brain Research. 2006 ; Vol. 1085, No. 1. pp. 177-182.
@article{e7bdcf78ce4f417d8dd5cc55f278cca2,
title = "Expression of thymosin β in the rat brain following transient global ischemia",
abstract = "Thymosin β (Tβ) isoforms play an important role in the organization of the cytoskeleton by sequestering G-actin during development of the mammalian brain. In this study, we examined changes in the expression of Tβ4 and Tβ15 after transient global ischemia. Tβ15 mRNA increased gradually in the dentate gyrus (DG) of the hippocampal formation from 3 h after reperfusion and peaked 9 h later. Similarly, a significant increase in Tβ4 mRNA level was observed in the DG 12 h after reperfusion. Tβ4 and Tβ15 proteins were found in different cell types in control brains; Tβ15 was expressed in a subset of doublecortin (DCX)-positive cells in the DG, whereas Tβ4-IR was observed in DG neurons and nearby microglial cells. After ischemia, Tβ15-IR was found in DG neurons and Tβ4-IR in the reactivated microglial cells. Interestingly, Tβ15-IR accumulated in the nuclei of CA1 neurons, which are vulnerable to ischemic insults. These results suggest that Tβ4 and Tβ15 function in different cellular contexts during ischemia-induced responses.",
keywords = "Actin cytoskeleton, Ischemia, Nuclear accumulation, Thymosin beta",
author = "Younghwa Kim and Kim, {Eun Hae} and Soontaek Hong and Rhyu, {Im Joo} and Jeehyung Choe and Woong Sun and Hyun Kim",
year = "2006",
month = "4",
day = "26",
doi = "10.1016/j.brainres.2006.01.065",
language = "English",
volume = "1085",
pages = "177--182",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Expression of thymosin β in the rat brain following transient global ischemia

AU - Kim, Younghwa

AU - Kim, Eun Hae

AU - Hong, Soontaek

AU - Rhyu, Im Joo

AU - Choe, Jeehyung

AU - Sun, Woong

AU - Kim, Hyun

PY - 2006/4/26

Y1 - 2006/4/26

N2 - Thymosin β (Tβ) isoforms play an important role in the organization of the cytoskeleton by sequestering G-actin during development of the mammalian brain. In this study, we examined changes in the expression of Tβ4 and Tβ15 after transient global ischemia. Tβ15 mRNA increased gradually in the dentate gyrus (DG) of the hippocampal formation from 3 h after reperfusion and peaked 9 h later. Similarly, a significant increase in Tβ4 mRNA level was observed in the DG 12 h after reperfusion. Tβ4 and Tβ15 proteins were found in different cell types in control brains; Tβ15 was expressed in a subset of doublecortin (DCX)-positive cells in the DG, whereas Tβ4-IR was observed in DG neurons and nearby microglial cells. After ischemia, Tβ15-IR was found in DG neurons and Tβ4-IR in the reactivated microglial cells. Interestingly, Tβ15-IR accumulated in the nuclei of CA1 neurons, which are vulnerable to ischemic insults. These results suggest that Tβ4 and Tβ15 function in different cellular contexts during ischemia-induced responses.

AB - Thymosin β (Tβ) isoforms play an important role in the organization of the cytoskeleton by sequestering G-actin during development of the mammalian brain. In this study, we examined changes in the expression of Tβ4 and Tβ15 after transient global ischemia. Tβ15 mRNA increased gradually in the dentate gyrus (DG) of the hippocampal formation from 3 h after reperfusion and peaked 9 h later. Similarly, a significant increase in Tβ4 mRNA level was observed in the DG 12 h after reperfusion. Tβ4 and Tβ15 proteins were found in different cell types in control brains; Tβ15 was expressed in a subset of doublecortin (DCX)-positive cells in the DG, whereas Tβ4-IR was observed in DG neurons and nearby microglial cells. After ischemia, Tβ15-IR was found in DG neurons and Tβ4-IR in the reactivated microglial cells. Interestingly, Tβ15-IR accumulated in the nuclei of CA1 neurons, which are vulnerable to ischemic insults. These results suggest that Tβ4 and Tβ15 function in different cellular contexts during ischemia-induced responses.

KW - Actin cytoskeleton

KW - Ischemia

KW - Nuclear accumulation

KW - Thymosin beta

UR - http://www.scopus.com/inward/record.url?scp=33646755391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646755391&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2006.01.065

DO - 10.1016/j.brainres.2006.01.065

M3 - Article

VL - 1085

SP - 177

EP - 182

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -