Expression of TNF-alpha and TGF-beta 1 in the rat brain after a single high-dose irradiation.

Se-Hoon Kim, Dong Jun Lim, Yong Gu Chung, Tai Hyoung Cho, Seong Jun Lim, Woo Jae Kim, Jung Keun Suh

Research output: Contribution to journalArticle

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Abstract

Cytokines and growth factors are important regulatory proteins controlling the growth and differentiation of normal and malignant glial cells. In this study, we investigated the expression and origin of tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta 1 (TGF-beta 1) in the subacute brain injury after a single high-dose irradiation using 60 Sprague-Dawley rats. The right cerebral hemispheres of rats were exposed to a single 10 Gy dose of gamma rays using Ir-192. The radiation effect was assessed at 1 week, 2 weeks, 4 weeks, 6 weeks, and 8 weeks after irradiation, and the results were compared with those in sham operation group. Histological changes characteristic of radiation injury were correlated with the duration after the single dose irradiation. The loss of cortical thickness also increased with the lapse of time after irradiation. The TNF-alpha expression in the irradiated cerebral hemispheres was significantly increased compared with that in the sham operation group. TGF-beta 1 expression was also increased in the irradiated hemispheres. Immunohistochemical study revealed that TGF-beta 1 was expressed predominantly by infiltrating macrophages and astrocytes around the necrotic areas. These findings indicate that TNF-alpha and TGF-beta 1 may play prominent roles in the radiation injuries after a single high-dose irradiation.

Original languageEnglish
Pages (from-to)242-248
Number of pages7
JournalJournal of Korean Medical Science
Volume17
Issue number2
Publication statusPublished - 2002 Apr 1

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Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Radiation Injuries
Brain
Cerebrum
Gamma Rays
Radiation Effects
Neuroglia
Astrocytes
Brain Injuries
Sprague Dawley Rats
Intercellular Signaling Peptides and Proteins
Macrophages
Cytokines
rat Tgfb1 protein
Growth
Proteins

ASJC Scopus subject areas

  • Medicine(all)

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Expression of TNF-alpha and TGF-beta 1 in the rat brain after a single high-dose irradiation. / Kim, Se-Hoon; Lim, Dong Jun; Chung, Yong Gu; Cho, Tai Hyoung; Lim, Seong Jun; Kim, Woo Jae; Suh, Jung Keun.

In: Journal of Korean Medical Science, Vol. 17, No. 2, 01.04.2002, p. 242-248.

Research output: Contribution to journalArticle

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