Expression of transforming growth factor-β-inducible gene-h3 in normal and cyclosporine-treated rat kidney

Bo Kyung Sun, Can Li, Sun Woo Lim, Ju Young Jung, Suk Hee Lee, In-San Kim, Yong Soo Kim, Jin Kim, Byung Kee Bang, Chul Woo Yang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Up-regulation of transforming growth factor-β (TGF-β) is known to play an important role in the tubulointerstitial injury of chronic cyclosporin A (CsA) nephropathy, but the expression of the TGF-β-inducible gane-h3 (βig-h3) is undetermined. In this study we examined βig-h3 expression and its relationship to tubulointerstitial injury in a rat model of chronic CsA nephropathy. Sprague-Dawley rats kept on a low-salt diet (0.05% sodium) were treated daily for 4 weeks with subcutaneous injections of vehicle (olive oil, 1 mL/kg) or CsA (15 mg/kg). The expression of βig-h3 messenger RNA (mRNA) and protein was evaluated with the use of in situ hybridization, immunohistochemical methods, and immunoblotting. We also compared renal function, histologic findings (tubulointerstitial fibrosis), and expression of TGF-β1 among treatment groups. In vehicle-treated kidney, βig-h3 mRNA and protein were constitutively expressed in the outer medulla and cortex, which was confined to the terminal portion of afferent arterioles, the S3 segment of the proximal tubules, and distal convoluted tubules. CsA treatment significantly up-regulated βig-h3 expression in the interstitium, especially in expanded and fibrotic areas. Quantitative analysis revealed that CsA induced a significant (two-fold) increase in βig-h3 mRNA and protein, and this increase was correlated with up-regulation of TGF-β1 expression (r = .943, P < .001) and the tubulointerstitial fibrosis score (r = .746, P = .05). Our observations indicate that an increase in βig-h3 expression, along with TGF-β1 up-regulation, is closely associated with tubulointerstitial fibrosis in a rat model of chronic CsA nephropathy.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalJournal of Laboratory and Clinical Medicine
Volume143
Issue number3
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Transforming Growth Factors
Cyclosporine
Rats
Genes
Kidney
Fibrosis
Up-Regulation
Messenger RNA
Sodium-Restricted Diet
Proteins
Wounds and Injuries
Arterioles
Subcutaneous Injections
Nutrition
Immunoblotting
In Situ Hybridization
Sprague Dawley Rats
Salts
Sodium
Therapeutics

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of transforming growth factor-β-inducible gene-h3 in normal and cyclosporine-treated rat kidney. / Sun, Bo Kyung; Li, Can; Lim, Sun Woo; Jung, Ju Young; Lee, Suk Hee; Kim, In-San; Kim, Yong Soo; Kim, Jin; Bang, Byung Kee; Yang, Chul Woo.

In: Journal of Laboratory and Clinical Medicine, Vol. 143, No. 3, 01.01.2004, p. 175-183.

Research output: Contribution to journalArticle

Sun, Bo Kyung ; Li, Can ; Lim, Sun Woo ; Jung, Ju Young ; Lee, Suk Hee ; Kim, In-San ; Kim, Yong Soo ; Kim, Jin ; Bang, Byung Kee ; Yang, Chul Woo. / Expression of transforming growth factor-β-inducible gene-h3 in normal and cyclosporine-treated rat kidney. In: Journal of Laboratory and Clinical Medicine. 2004 ; Vol. 143, No. 3. pp. 175-183.
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AU - Lee, Suk Hee

AU - Kim, In-San

AU - Kim, Yong Soo

AU - Kim, Jin

AU - Bang, Byung Kee

AU - Yang, Chul Woo

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