Expression of vascular endothelial growth factor in otitis media

Hak Hyun Jung, Myung Won Kim, Jae Hyuck Lee, Young Tae Kim, Nan Hee Kim, Baik Ahm Chang, Jong Ouck Choi, Hyun Ho Lim

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Increased vascular permeability and endothelial cell growth are important in the pathogenesis of otitis media with effusion (OME) and the vascular endothelial growth factor (VEGF) is known to play an important role in the increased vascular permeability and angiogenesis. To date, at least five isoforms of the VEGF family have been identified as VEGF transcripts, encoding polypeptides of 206, 189, 165, 145 and 121, but their physiological roles are unclear. The purpose of this study was to investigate the expression of VEGF, in both endotoxin-induced OME of the rat and human otitis media. We instilled endotoxin and saline as a control into the middle ear cavity of the rat. Middle ear mucosa were taken at 0 h, 1 h, 3 h, 6 h, 12 h, 1 day, 3 days, 7 days and 14 days and the expression of VEGF mRNA and VEGF protein was evaluated using semi-quantitative RT-PCR and immunohistochemistry. Expression of VEGF164 mRNA and VEGF120 mRNA was first identified 1 h after endotoxin instillation and was dramatically increased over the period 6 h-1 day and then progressively decreased by day 7. The level of expression of VEGF120 mRNA was slightly higher than that of VEGF164 mRNA and that of VEGF164 mRNA was much higher than that of VEGF188 mRNA. Immunostaining revealed expression of VEGF during 6 h to day 3 and its expression was localized to ciliated cells and some inflammatory cells. We also performed RT-PCRs of cDNA from middle ear fluids of 8 human OME patients and middle ear mucosa of 4 chronic otitis media patients for the identification of VEGF mRNA expression. VEGF121 mRNA was highly expressed in all samples compared with VEGF165 mRNA. These results suggest that VEGF may be primarily responsible for increased vascular permeability and endothelial cell growth in OME and that VEGF seems to play a significant role in the pathogenesis of OME.

Original languageEnglish
Pages (from-to)801-808
Number of pages8
JournalActa Oto-Laryngologica
Volume119
Issue number7
Publication statusPublished - 1999 Dec 1

Fingerprint

Otitis Media
Vascular Endothelial Growth Factor A
Messenger RNA
Otitis Media with Effusion
Middle Ear
Capillary Permeability
Endotoxins
Mucous Membrane
Endothelial Cells
Polymerase Chain Reaction
Growth
Protein Isoforms
Complementary DNA
Immunohistochemistry
Peptides

Keywords

  • Gene expression
  • Otitis media with effusion (OME)
  • Polymerase chain reaction
  • Rat
  • Vascular permeability

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Jung, H. H., Kim, M. W., Lee, J. H., Kim, Y. T., Kim, N. H., Chang, B. A., ... Lim, H. H. (1999). Expression of vascular endothelial growth factor in otitis media. Acta Oto-Laryngologica, 119(7), 801-808.

Expression of vascular endothelial growth factor in otitis media. / Jung, Hak Hyun; Kim, Myung Won; Lee, Jae Hyuck; Kim, Young Tae; Kim, Nan Hee; Chang, Baik Ahm; Choi, Jong Ouck; Lim, Hyun Ho.

In: Acta Oto-Laryngologica, Vol. 119, No. 7, 01.12.1999, p. 801-808.

Research output: Contribution to journalArticle

Jung, HH, Kim, MW, Lee, JH, Kim, YT, Kim, NH, Chang, BA, Choi, JO & Lim, HH 1999, 'Expression of vascular endothelial growth factor in otitis media', Acta Oto-Laryngologica, vol. 119, no. 7, pp. 801-808.
Jung HH, Kim MW, Lee JH, Kim YT, Kim NH, Chang BA et al. Expression of vascular endothelial growth factor in otitis media. Acta Oto-Laryngologica. 1999 Dec 1;119(7):801-808.
Jung, Hak Hyun ; Kim, Myung Won ; Lee, Jae Hyuck ; Kim, Young Tae ; Kim, Nan Hee ; Chang, Baik Ahm ; Choi, Jong Ouck ; Lim, Hyun Ho. / Expression of vascular endothelial growth factor in otitis media. In: Acta Oto-Laryngologica. 1999 ; Vol. 119, No. 7. pp. 801-808.
@article{b9d60e34996d4409b29d3c128205d8ba,
title = "Expression of vascular endothelial growth factor in otitis media",
abstract = "Increased vascular permeability and endothelial cell growth are important in the pathogenesis of otitis media with effusion (OME) and the vascular endothelial growth factor (VEGF) is known to play an important role in the increased vascular permeability and angiogenesis. To date, at least five isoforms of the VEGF family have been identified as VEGF transcripts, encoding polypeptides of 206, 189, 165, 145 and 121, but their physiological roles are unclear. The purpose of this study was to investigate the expression of VEGF, in both endotoxin-induced OME of the rat and human otitis media. We instilled endotoxin and saline as a control into the middle ear cavity of the rat. Middle ear mucosa were taken at 0 h, 1 h, 3 h, 6 h, 12 h, 1 day, 3 days, 7 days and 14 days and the expression of VEGF mRNA and VEGF protein was evaluated using semi-quantitative RT-PCR and immunohistochemistry. Expression of VEGF164 mRNA and VEGF120 mRNA was first identified 1 h after endotoxin instillation and was dramatically increased over the period 6 h-1 day and then progressively decreased by day 7. The level of expression of VEGF120 mRNA was slightly higher than that of VEGF164 mRNA and that of VEGF164 mRNA was much higher than that of VEGF188 mRNA. Immunostaining revealed expression of VEGF during 6 h to day 3 and its expression was localized to ciliated cells and some inflammatory cells. We also performed RT-PCRs of cDNA from middle ear fluids of 8 human OME patients and middle ear mucosa of 4 chronic otitis media patients for the identification of VEGF mRNA expression. VEGF121 mRNA was highly expressed in all samples compared with VEGF165 mRNA. These results suggest that VEGF may be primarily responsible for increased vascular permeability and endothelial cell growth in OME and that VEGF seems to play a significant role in the pathogenesis of OME.",
keywords = "Gene expression, Otitis media with effusion (OME), Polymerase chain reaction, Rat, Vascular permeability",
author = "Jung, {Hak Hyun} and Kim, {Myung Won} and Lee, {Jae Hyuck} and Kim, {Young Tae} and Kim, {Nan Hee} and Chang, {Baik Ahm} and Choi, {Jong Ouck} and Lim, {Hyun Ho}",
year = "1999",
month = "12",
day = "1",
language = "English",
volume = "119",
pages = "801--808",
journal = "Acta Oto-Laryngologica",
issn = "0001-6489",
publisher = "Informa Healthcare",
number = "7",

}

TY - JOUR

T1 - Expression of vascular endothelial growth factor in otitis media

AU - Jung, Hak Hyun

AU - Kim, Myung Won

AU - Lee, Jae Hyuck

AU - Kim, Young Tae

AU - Kim, Nan Hee

AU - Chang, Baik Ahm

AU - Choi, Jong Ouck

AU - Lim, Hyun Ho

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Increased vascular permeability and endothelial cell growth are important in the pathogenesis of otitis media with effusion (OME) and the vascular endothelial growth factor (VEGF) is known to play an important role in the increased vascular permeability and angiogenesis. To date, at least five isoforms of the VEGF family have been identified as VEGF transcripts, encoding polypeptides of 206, 189, 165, 145 and 121, but their physiological roles are unclear. The purpose of this study was to investigate the expression of VEGF, in both endotoxin-induced OME of the rat and human otitis media. We instilled endotoxin and saline as a control into the middle ear cavity of the rat. Middle ear mucosa were taken at 0 h, 1 h, 3 h, 6 h, 12 h, 1 day, 3 days, 7 days and 14 days and the expression of VEGF mRNA and VEGF protein was evaluated using semi-quantitative RT-PCR and immunohistochemistry. Expression of VEGF164 mRNA and VEGF120 mRNA was first identified 1 h after endotoxin instillation and was dramatically increased over the period 6 h-1 day and then progressively decreased by day 7. The level of expression of VEGF120 mRNA was slightly higher than that of VEGF164 mRNA and that of VEGF164 mRNA was much higher than that of VEGF188 mRNA. Immunostaining revealed expression of VEGF during 6 h to day 3 and its expression was localized to ciliated cells and some inflammatory cells. We also performed RT-PCRs of cDNA from middle ear fluids of 8 human OME patients and middle ear mucosa of 4 chronic otitis media patients for the identification of VEGF mRNA expression. VEGF121 mRNA was highly expressed in all samples compared with VEGF165 mRNA. These results suggest that VEGF may be primarily responsible for increased vascular permeability and endothelial cell growth in OME and that VEGF seems to play a significant role in the pathogenesis of OME.

AB - Increased vascular permeability and endothelial cell growth are important in the pathogenesis of otitis media with effusion (OME) and the vascular endothelial growth factor (VEGF) is known to play an important role in the increased vascular permeability and angiogenesis. To date, at least five isoforms of the VEGF family have been identified as VEGF transcripts, encoding polypeptides of 206, 189, 165, 145 and 121, but their physiological roles are unclear. The purpose of this study was to investigate the expression of VEGF, in both endotoxin-induced OME of the rat and human otitis media. We instilled endotoxin and saline as a control into the middle ear cavity of the rat. Middle ear mucosa were taken at 0 h, 1 h, 3 h, 6 h, 12 h, 1 day, 3 days, 7 days and 14 days and the expression of VEGF mRNA and VEGF protein was evaluated using semi-quantitative RT-PCR and immunohistochemistry. Expression of VEGF164 mRNA and VEGF120 mRNA was first identified 1 h after endotoxin instillation and was dramatically increased over the period 6 h-1 day and then progressively decreased by day 7. The level of expression of VEGF120 mRNA was slightly higher than that of VEGF164 mRNA and that of VEGF164 mRNA was much higher than that of VEGF188 mRNA. Immunostaining revealed expression of VEGF during 6 h to day 3 and its expression was localized to ciliated cells and some inflammatory cells. We also performed RT-PCRs of cDNA from middle ear fluids of 8 human OME patients and middle ear mucosa of 4 chronic otitis media patients for the identification of VEGF mRNA expression. VEGF121 mRNA was highly expressed in all samples compared with VEGF165 mRNA. These results suggest that VEGF may be primarily responsible for increased vascular permeability and endothelial cell growth in OME and that VEGF seems to play a significant role in the pathogenesis of OME.

KW - Gene expression

KW - Otitis media with effusion (OME)

KW - Polymerase chain reaction

KW - Rat

KW - Vascular permeability

UR - http://www.scopus.com/inward/record.url?scp=0033395318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033395318&partnerID=8YFLogxK

M3 - Article

C2 - 10687938

AN - SCOPUS:0033395318

VL - 119

SP - 801

EP - 808

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

IS - 7

ER -