Expression of vascular endothelial growth factor in response to high glucose in rat mesangial cells

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Abstract

Diabetic nephropathy associated with hyperglycemia is characterized by glomerular hyperfiltration and endothelial dysfunction. Vascular endothelial growth factor (VEGF) is known to be primarily involved in neoangiogenesis and increased endothelial permeability. The purpose of this study was to investigate VEGF expression in response to high glucose in rat cultured mesangial cells and to identify its signal pathway via protein kinase C (PKC). Rat mesangial cells were cultured with different concentrations of glucose: normal (5 mM D-glucose), medium (15 mM D-glucose) and high (30 mm D- glucose). Calphostin-C as a PKC inhibitor and phorbol myristate acetate (PMA) as a PKC downregulator were instillated into culture media to evaluate the role of PKC in mediating the glucose- induced increase in VEGF expression. High glucose increased expression of VEGF at the mRNA and protein levels, identified by semi-quantitative RT-PCR and western blotting, within 3 h and in a time- and glucose concentration-dependent manner. Calphostin-C and PMA inhibited glucose-induced increases in VEGF expression at the mRNA and protein levels. In conclusion, high glucose can directly increase VEGF expression in rat mesangial cells via a PKC-dependent mechanism. These results suggest that VEGF could be a potential mediator of glomerular hyperfiltration and proteinuria in diabetic nephropathy.

Original languageEnglish
Pages (from-to)617-624
Number of pages8
JournalJournal of Endocrinology
Volume165
Issue number3
DOIs
Publication statusPublished - 2000 Jan 1

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Mesangial Cells
Vascular Endothelial Growth Factor A
Glucose
Protein Kinase C
Diabetic Nephropathies
Tetradecanoylphorbol Acetate
Messenger RNA
Protein C Inhibitor
Protein Kinase Inhibitors
Proteinuria
Hyperglycemia
Culture Media
Cultured Cells
Permeability
Signal Transduction
Proteins
Western Blotting
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Expression of vascular endothelial growth factor in response to high glucose in rat mesangial cells. / Kim, Nan Hee; Jung, Hak Hyun; Cha, Dae-Ryong; Choi, D. S.

In: Journal of Endocrinology, Vol. 165, No. 3, 01.01.2000, p. 617-624.

Research output: Contribution to journalArticle

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