Expression of vascular endothelial growth factor in response to high glucose in rat mesangial cells

N. H. Kim, H. H. Jung, D. R. Cha, D. S. Choi

    Research output: Contribution to journalArticlepeer-review

    70 Citations (Scopus)

    Abstract

    Diabetic nephropathy associated with hyperglycemia is characterized by glomerular hyperfiltration and endothelial dysfunction. Vascular endothelial growth factor (VEGF) is known to be primarily involved in neoangiogenesis and increased endothelial permeability. The purpose of this study was to investigate VEGF expression in response to high glucose in rat cultured mesangial cells and to identify its signal pathway via protein kinase C (PKC). Rat mesangial cells were cultured with different concentrations of glucose: normal (5 mM D-glucose), medium (15 mM D-glucose) and high (30 mm D- glucose). Calphostin-C as a PKC inhibitor and phorbol myristate acetate (PMA) as a PKC downregulator were instillated into culture media to evaluate the role of PKC in mediating the glucose- induced increase in VEGF expression. High glucose increased expression of VEGF at the mRNA and protein levels, identified by semi-quantitative RT-PCR and western blotting, within 3 h and in a time- and glucose concentration-dependent manner. Calphostin-C and PMA inhibited glucose-induced increases in VEGF expression at the mRNA and protein levels. In conclusion, high glucose can directly increase VEGF expression in rat mesangial cells via a PKC-dependent mechanism. These results suggest that VEGF could be a potential mediator of glomerular hyperfiltration and proteinuria in diabetic nephropathy.

    Original languageEnglish
    Pages (from-to)617-624
    Number of pages8
    JournalJournal of Endocrinology
    Volume165
    Issue number3
    DOIs
    Publication statusPublished - 2000

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Endocrinology

    Fingerprint

    Dive into the research topics of 'Expression of vascular endothelial growth factor in response to high glucose in rat mesangial cells'. Together they form a unique fingerprint.

    Cite this