Expression pattern of the class I homeobox genes in ovarian carcinoma

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis. Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries. Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries. Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalJournal of Gynecologic Oncology
Volume21
Issue number1
DOIs
Publication statusPublished - 2010 Mar 1

Fingerprint

MHC Class I Genes
Homeobox Genes
Ovarian Neoplasms
Carcinoma
Ovary
Cell Line
Genes
Carcinogenesis
Western Blotting
Reverse Transcription
Neoplasms
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Carcinogenesis
  • Homeobox gene
  • Ovarian neoplasms

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

Cite this

Expression pattern of the class I homeobox genes in ovarian carcinoma. / Hong, Jin-Hwa; Lee, Jae Kwan; Park, Joong Jean; Lee, Nak Woo; Lee, Kyu Wan; Na, Jung Yeol.

In: Journal of Gynecologic Oncology, Vol. 21, No. 1, 01.03.2010, p. 29-37.

Research output: Contribution to journalArticle

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