Expression pattern of the class I homeobox genes in ovarian carcinoma

Jin Hwa Hong, Jae Kwan Lee, Joong Jean Park, Nak Woo Lee, Kyu Wan Lee, Jung Yeol Na

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis. Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries. Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries. Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalJournal of gynecologic oncology
Volume21
Issue number1
DOIs
Publication statusPublished - 2010 Mar

Keywords

  • Carcinogenesis
  • Homeobox gene
  • Ovarian neoplasms

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

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  • Cite this

    Hong, J. H., Lee, J. K., Park, J. J., Lee, N. W., Lee, K. W., & Na, J. Y. (2010). Expression pattern of the class I homeobox genes in ovarian carcinoma. Journal of gynecologic oncology, 21(1), 29-37. https://doi.org/10.3802/jgo.2010.21.1.29