Abstract
Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis. Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries. Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries. Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 29-37 |
| Number of pages | 9 |
| Journal | Journal of Gynecologic Oncology |
| Volume | 21 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 Mar 1 |
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Keywords
- Carcinogenesis
- Homeobox gene
- Ovarian neoplasms
ASJC Scopus subject areas
- Oncology
- Obstetrics and Gynaecology
Cite this
Expression pattern of the class I homeobox genes in ovarian carcinoma. / Hong, Jin-Hwa; Lee, Jae Kwan; Park, Joong Jean; Lee, Nak Woo; Lee, Kyu Wan; Na, Jung Yeol.
In: Journal of Gynecologic Oncology, Vol. 21, No. 1, 01.03.2010, p. 29-37.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression pattern of the class I homeobox genes in ovarian carcinoma
AU - Hong, Jin-Hwa
AU - Lee, Jae Kwan
AU - Park, Joong Jean
AU - Lee, Nak Woo
AU - Lee, Kyu Wan
AU - Na, Jung Yeol
PY - 2010/3/1
Y1 - 2010/3/1
N2 - Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis. Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries. Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries. Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.
AB - Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis. Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries. Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries. Conclusion: We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.
KW - Carcinogenesis
KW - Homeobox gene
KW - Ovarian neoplasms
UR - http://www.scopus.com/inward/record.url?scp=77953428228&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953428228&partnerID=8YFLogxK
U2 - 10.3802/jgo.2010.21.1.29
DO - 10.3802/jgo.2010.21.1.29
M3 - Article
C2 - 20379445
AN - SCOPUS:77953428228
VL - 21
SP - 29
EP - 37
JO - Journal of Gynecologic Oncology
JF - Journal of Gynecologic Oncology
SN - 2005-0380
IS - 1
ER -