Expression patterns of βig-h3 in chondrocyte differentiation during endochondral ossification

Min Su Han, Jung Eun Kim, Hong In Shin, In-San Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

βig-h3 is a TGF-β-induced extracellular matrix protein which is expressed in many tissues including bones and cartilages. In previous reports, we showed that βig-h3 mediates cell adhesion and migration and, especially in bones, negatively regulates the mineralization in the end stage of endochondral ossification. Here, to elucidate the expression pattern and role of βig-h3 in chondrocyte differentiation, ATDC5 chondrocytes and embryonic and postnatal mice were used for in vitro differentiation studies and in vivo studies, respectively. βig-h3 was strongly induced by the treatment of TGF-β1 and the expression level of βig-h3 mRNA and protein were highly expressed in the early stages of differentiation but decreased in the late stages in ATDC5. Furthermore, the patterns of TGF-β1, -β2, and -β3 mRNA expression were concurrent with βig-h3 in ATDC5. βig-h3 was deeply stained in perichondrium (PC), periosteum (PO), and prehypertro-phic chondrocytes (PH) through the entire period of endochondral ossification in mice. βig-h3 was mainly expressed in PC and PH at embryonic days and obviously in PH in postnatal days. These results suggest that βig-h3 may play a critical role as a regulator of chondrogenic differentiation in endochondral ossification.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalExperimental and Molecular Medicine
Volume40
Issue number4
DOIs
Publication statusPublished - 2008 Aug 31
Externally publishedYes

Keywords

  • Cell differentiation
  • Chondrocytes
  • Osteogenesis
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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