Abstract
βig-h3 is a TGF-β-induced extracellular matrix protein which is expressed in many tissues including bones and cartilages. In previous reports, we showed that βig-h3 mediates cell adhesion and migration and, especially in bones, negatively regulates the mineralization in the end stage of endochondral ossification. Here, to elucidate the expression pattern and role of βig-h3 in chondrocyte differentiation, ATDC5 chondrocytes and embryonic and postnatal mice were used for in vitro differentiation studies and in vivo studies, respectively. βig-h3 was strongly induced by the treatment of TGF-β1 and the expression level of βig-h3 mRNA and protein were highly expressed in the early stages of differentiation but decreased in the late stages in ATDC5. Furthermore, the patterns of TGF-β1, -β2, and -β3 mRNA expression were concurrent with βig-h3 in ATDC5. βig-h3 was deeply stained in perichondrium (PC), periosteum (PO), and prehypertro-phic chondrocytes (PH) through the entire period of endochondral ossification in mice. βig-h3 was mainly expressed in PC and PH at embryonic days and obviously in PH in postnatal days. These results suggest that βig-h3 may play a critical role as a regulator of chondrogenic differentiation in endochondral ossification.
| Original language | English |
|---|---|
| Pages (from-to) | 453-460 |
| Number of pages | 8 |
| Journal | Experimental and Molecular Medicine |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 Aug 31 |
| Externally published | Yes |
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Keywords
- Cell differentiation
- Chondrocytes
- Osteogenesis
- Transforming growth factor-β
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Clinical Biochemistry
Cite this
Expression patterns of βig-h3 in chondrocyte differentiation during endochondral ossification. / Han, Min Su; Kim, Jung Eun; Shin, Hong In; Kim, In-San.
In: Experimental and Molecular Medicine, Vol. 40, No. 4, 31.08.2008, p. 453-460.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression patterns of βig-h3 in chondrocyte differentiation during endochondral ossification
AU - Han, Min Su
AU - Kim, Jung Eun
AU - Shin, Hong In
AU - Kim, In-San
PY - 2008/8/31
Y1 - 2008/8/31
N2 - βig-h3 is a TGF-β-induced extracellular matrix protein which is expressed in many tissues including bones and cartilages. In previous reports, we showed that βig-h3 mediates cell adhesion and migration and, especially in bones, negatively regulates the mineralization in the end stage of endochondral ossification. Here, to elucidate the expression pattern and role of βig-h3 in chondrocyte differentiation, ATDC5 chondrocytes and embryonic and postnatal mice were used for in vitro differentiation studies and in vivo studies, respectively. βig-h3 was strongly induced by the treatment of TGF-β1 and the expression level of βig-h3 mRNA and protein were highly expressed in the early stages of differentiation but decreased in the late stages in ATDC5. Furthermore, the patterns of TGF-β1, -β2, and -β3 mRNA expression were concurrent with βig-h3 in ATDC5. βig-h3 was deeply stained in perichondrium (PC), periosteum (PO), and prehypertro-phic chondrocytes (PH) through the entire period of endochondral ossification in mice. βig-h3 was mainly expressed in PC and PH at embryonic days and obviously in PH in postnatal days. These results suggest that βig-h3 may play a critical role as a regulator of chondrogenic differentiation in endochondral ossification.
AB - βig-h3 is a TGF-β-induced extracellular matrix protein which is expressed in many tissues including bones and cartilages. In previous reports, we showed that βig-h3 mediates cell adhesion and migration and, especially in bones, negatively regulates the mineralization in the end stage of endochondral ossification. Here, to elucidate the expression pattern and role of βig-h3 in chondrocyte differentiation, ATDC5 chondrocytes and embryonic and postnatal mice were used for in vitro differentiation studies and in vivo studies, respectively. βig-h3 was strongly induced by the treatment of TGF-β1 and the expression level of βig-h3 mRNA and protein were highly expressed in the early stages of differentiation but decreased in the late stages in ATDC5. Furthermore, the patterns of TGF-β1, -β2, and -β3 mRNA expression were concurrent with βig-h3 in ATDC5. βig-h3 was deeply stained in perichondrium (PC), periosteum (PO), and prehypertro-phic chondrocytes (PH) through the entire period of endochondral ossification in mice. βig-h3 was mainly expressed in PC and PH at embryonic days and obviously in PH in postnatal days. These results suggest that βig-h3 may play a critical role as a regulator of chondrogenic differentiation in endochondral ossification.
KW - Cell differentiation
KW - Chondrocytes
KW - Osteogenesis
KW - Transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=51949095469&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=51949095469&partnerID=8YFLogxK
U2 - 10.3858/emm.2008.40.4.453
DO - 10.3858/emm.2008.40.4.453
M3 - Article
C2 - 18779658
AN - SCOPUS:51949095469
VL - 40
SP - 453
EP - 460
JO - Experimental and Molecular Medicine
JF - Experimental and Molecular Medicine
SN - 1226-3613
IS - 4
ER -