Expression profiles of p53-, p16INK4a-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells

Hyunggee Kim, Seungkwon You, James Farris, Byung Whi Kong, Shelly A. Christman, Linda K. Foster, Douglas N. Foster

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Replicative senescence is known to be an intrinsic mechanism in determining the finite life span of in vitro cultured cells. Since this process is recognized as an evolutionarily conserved mechanism from yeast to mammalian cells, we compared the senescence-associated genetic alterations in the p53, p16INK4a, and telomere regulatory pathways using replicative senescent human, mouse, and chicken fibroblast cells. Normal human diploid fibroblast (HDF; WI38) and chicken embryonic fibroblast (CEF) cells were shown to have a more extended in vitro proliferative potential than their mouse embryonic fibroblast (MEF) counterpart. In contrast to the HDF and CEF cells, MEF cells were shown to express telomerase mRNA and maintain telomerase activity throughout their in vitro life span. Functional p53 activity was shown to increase in the replicative senescent HDF and CEF cells, but not in replicative senescent MEF cells. On the other hand, there was a gradual elevation of p16INK4a expression with increased cell passages which reached a maximum in replicative senescent MEF cells. Taken together, the present study demonstrates that the p53, p16INK4a, and telomere regulatory functions may be differentially regulated during replicative senescence in human, mouse, and chicken fibroblast cells.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalExperimental Cell Research
Volume272
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • CEF
  • HDF
  • MEF
  • Replicative senescence
  • Telomere
  • p16
  • p53

ASJC Scopus subject areas

  • Cell Biology

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