Expression profiles of p53-, p16INK4a-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells

Hyunggee Kim, Seungkwon You, James Farris, Byung Whi Kong, Shelly A. Christman, Linda K. Foster, Douglas N. Foster

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Replicative senescence is known to be an intrinsic mechanism in determining the finite life span of in vitro cultured cells. Since this process is recognized as an evolutionarily conserved mechanism from yeast to mammalian cells, we compared the senescence-associated genetic alterations in the p53, p16INK4a, and telomere regulatory pathways using replicative senescent human, mouse, and chicken fibroblast cells. Normal human diploid fibroblast (HDF; WI38) and chicken embryonic fibroblast (CEF) cells were shown to have a more extended in vitro proliferative potential than their mouse embryonic fibroblast (MEF) counterpart. In contrast to the HDF and CEF cells, MEF cells were shown to express telomerase mRNA and maintain telomerase activity throughout their in vitro life span. Functional p53 activity was shown to increase in the replicative senescent HDF and CEF cells, but not in replicative senescent MEF cells. On the other hand, there was a gradual elevation of p16INK4a expression with increased cell passages which reached a maximum in replicative senescent MEF cells. Taken together, the present study demonstrates that the p53, p16INK4a, and telomere regulatory functions may be differentially regulated during replicative senescence in human, mouse, and chicken fibroblast cells.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalExperimental Cell Research
Volume272
Issue number2
DOIs
Publication statusPublished - 2002 Sep 2

Fingerprint

Telomere
Chickens
Fibroblasts
Genes
Cell Aging
Telomerase
Diploidy
Cultured Cells
Yeasts
Messenger RNA

Keywords

  • CEF
  • HDF
  • MEF
  • p16
  • p53
  • Replicative senescence
  • Telomere

ASJC Scopus subject areas

  • Cell Biology

Cite this

Expression profiles of p53-, p16INK4a-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells. / Kim, Hyunggee; You, Seungkwon; Farris, James; Kong, Byung Whi; Christman, Shelly A.; Foster, Linda K.; Foster, Douglas N.

In: Experimental Cell Research, Vol. 272, No. 2, 02.09.2002, p. 199-208.

Research output: Contribution to journalArticle

Kim, Hyunggee ; You, Seungkwon ; Farris, James ; Kong, Byung Whi ; Christman, Shelly A. ; Foster, Linda K. ; Foster, Douglas N. / Expression profiles of p53-, p16INK4a-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells. In: Experimental Cell Research. 2002 ; Vol. 272, No. 2. pp. 199-208.
@article{213dc95663f247158eca33d5eefb7d06,
title = "Expression profiles of p53-, p16INK4a-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells",
abstract = "Replicative senescence is known to be an intrinsic mechanism in determining the finite life span of in vitro cultured cells. Since this process is recognized as an evolutionarily conserved mechanism from yeast to mammalian cells, we compared the senescence-associated genetic alterations in the p53, p16INK4a, and telomere regulatory pathways using replicative senescent human, mouse, and chicken fibroblast cells. Normal human diploid fibroblast (HDF; WI38) and chicken embryonic fibroblast (CEF) cells were shown to have a more extended in vitro proliferative potential than their mouse embryonic fibroblast (MEF) counterpart. In contrast to the HDF and CEF cells, MEF cells were shown to express telomerase mRNA and maintain telomerase activity throughout their in vitro life span. Functional p53 activity was shown to increase in the replicative senescent HDF and CEF cells, but not in replicative senescent MEF cells. On the other hand, there was a gradual elevation of p16INK4a expression with increased cell passages which reached a maximum in replicative senescent MEF cells. Taken together, the present study demonstrates that the p53, p16INK4a, and telomere regulatory functions may be differentially regulated during replicative senescence in human, mouse, and chicken fibroblast cells.",
keywords = "CEF, HDF, MEF, p16, p53, Replicative senescence, Telomere",
author = "Hyunggee Kim and Seungkwon You and James Farris and Kong, {Byung Whi} and Christman, {Shelly A.} and Foster, {Linda K.} and Foster, {Douglas N.}",
year = "2002",
month = "9",
day = "2",
doi = "10.1006/excr.2001.5420",
language = "English",
volume = "272",
pages = "199--208",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Expression profiles of p53-, p16INK4a-, and telomere-regulating genes in replicative senescent primary human, mouse, and chicken fibroblast cells

AU - Kim, Hyunggee

AU - You, Seungkwon

AU - Farris, James

AU - Kong, Byung Whi

AU - Christman, Shelly A.

AU - Foster, Linda K.

AU - Foster, Douglas N.

PY - 2002/9/2

Y1 - 2002/9/2

N2 - Replicative senescence is known to be an intrinsic mechanism in determining the finite life span of in vitro cultured cells. Since this process is recognized as an evolutionarily conserved mechanism from yeast to mammalian cells, we compared the senescence-associated genetic alterations in the p53, p16INK4a, and telomere regulatory pathways using replicative senescent human, mouse, and chicken fibroblast cells. Normal human diploid fibroblast (HDF; WI38) and chicken embryonic fibroblast (CEF) cells were shown to have a more extended in vitro proliferative potential than their mouse embryonic fibroblast (MEF) counterpart. In contrast to the HDF and CEF cells, MEF cells were shown to express telomerase mRNA and maintain telomerase activity throughout their in vitro life span. Functional p53 activity was shown to increase in the replicative senescent HDF and CEF cells, but not in replicative senescent MEF cells. On the other hand, there was a gradual elevation of p16INK4a expression with increased cell passages which reached a maximum in replicative senescent MEF cells. Taken together, the present study demonstrates that the p53, p16INK4a, and telomere regulatory functions may be differentially regulated during replicative senescence in human, mouse, and chicken fibroblast cells.

AB - Replicative senescence is known to be an intrinsic mechanism in determining the finite life span of in vitro cultured cells. Since this process is recognized as an evolutionarily conserved mechanism from yeast to mammalian cells, we compared the senescence-associated genetic alterations in the p53, p16INK4a, and telomere regulatory pathways using replicative senescent human, mouse, and chicken fibroblast cells. Normal human diploid fibroblast (HDF; WI38) and chicken embryonic fibroblast (CEF) cells were shown to have a more extended in vitro proliferative potential than their mouse embryonic fibroblast (MEF) counterpart. In contrast to the HDF and CEF cells, MEF cells were shown to express telomerase mRNA and maintain telomerase activity throughout their in vitro life span. Functional p53 activity was shown to increase in the replicative senescent HDF and CEF cells, but not in replicative senescent MEF cells. On the other hand, there was a gradual elevation of p16INK4a expression with increased cell passages which reached a maximum in replicative senescent MEF cells. Taken together, the present study demonstrates that the p53, p16INK4a, and telomere regulatory functions may be differentially regulated during replicative senescence in human, mouse, and chicken fibroblast cells.

KW - CEF

KW - HDF

KW - MEF

KW - p16

KW - p53

KW - Replicative senescence

KW - Telomere

UR - http://www.scopus.com/inward/record.url?scp=0036060157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036060157&partnerID=8YFLogxK

U2 - 10.1006/excr.2001.5420

DO - 10.1006/excr.2001.5420

M3 - Article

VL - 272

SP - 199

EP - 208

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 2

ER -