Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis

Joon Ryeol Yang, Eunjung Song, Byung G. Kim, Eung S. Kim, Jae Kyung Sohng, Min-Kyu Oh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Doxorubicin (DXR) and daunorubicin (DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. To improve their productivity, regulation of DXR/DNR synthesis genes as well as central metabolic pathway genes must be understood more clearly. So far, studies have focused on DXR/DNR gene regulation. To investigate the correlation between the central metabolic pathway genes and DXR/DNR productivity, we selected 265 genes involved in glycolysis, fermentation, the citric acid cycle, butanoate metabolism, etc., and searched for their sequences in the S. peucetius genome by comparing gene sequences to those of Streptomyces coelicolor. The homologous genes were amplified by PCR and arrayed on glass microarray slides. Gene expression was monitored under two different growth media conditions, R2YE and NDYE. Genes involved in the production of malonyl-CoA and propionyl-CoA, the main precursors for doxorubicin synthesis, were mainly upregulated in NDYE media. Genes related to acetyl-CoA and the urea cycle were also upregulated. These changes in gene expression were confirmed by real-time RT-PCR.

Original languageEnglish
Pages (from-to)738-744
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume13
Issue number6
DOIs
Publication statusPublished - 2008 Dec 1

Fingerprint

Streptomyces
Microarray Analysis
Microarrays
Oligonucleotide Array Sequence Analysis
DNA
Genes
Daunorubicin
Doxorubicin
Gene expression
Metabolic Networks and Pathways
Productivity
Malonyl Coenzyme A
Streptomyces coelicolor
Gene Expression
Acetyl Coenzyme A
Citric Acid Cycle
Anthracyclines
Glycolysis
Antibiotics
Metabolism

Keywords

  • DNA microarray
  • Doxorubicin
  • Metabolic gene
  • Streptomyces peucetius

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology

Cite this

Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis. / Yang, Joon Ryeol; Song, Eunjung; Kim, Byung G.; Kim, Eung S.; Sohng, Jae Kyung; Oh, Min-Kyu.

In: Biotechnology and Bioprocess Engineering, Vol. 13, No. 6, 01.12.2008, p. 738-744.

Research output: Contribution to journalArticle

Yang, Joon Ryeol ; Song, Eunjung ; Kim, Byung G. ; Kim, Eung S. ; Sohng, Jae Kyung ; Oh, Min-Kyu. / Expression profiling of Streptomyces peucetius metabolic genes using DNA microarray analysis. In: Biotechnology and Bioprocess Engineering. 2008 ; Vol. 13, No. 6. pp. 738-744.
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