Abstract
Regulator of G-protein signaling 5 (RGS5), an inhibitor of Gq and Gi activation, is a member of the small RGS protein subfamily. However, despite significant process in the investigation of RGS5, no structure is yet available. In order to elucidate the mechanism of the RGS5 in G protein signaling pathway, we have overexpressed the RGS5 and G_i3 from human in Escherichia coli and crystallized the complex of RGS5 and Gαi3 proteins with GDP/Mg2+/AlF4 - at 3.0 Å resolution using a synchrotron radiation source. The complex crystals belong to the tetragonal space group P41212 or P432 12, with unit cell parameters a=b=95.9 Å, and c=138.8 Å. Assuming one complex protein in the crystallographic asymmetric unit, the calculated Matthews parameter (VM) is 2.57 Å3/Da and solvent content is 52.2%.
| Original language | English |
|---|---|
| Pages (from-to) | 945-949 |
| Number of pages | 5 |
| Journal | Protein and Peptide Letters |
| Volume | 13 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2006 Sep 19 |
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Keywords
- Crystallization
- G protein signaling pathway
- Gαi
- Regulator of G-protein signaling 5
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Expression, purification, and preliminary X-ray crystallographic analysis of the complex of Gαi3-RGS5 from human with GDP/Mg 2+/AlF4 - . / Rhee, Kyung Hee; Nam, Ki Hyun; Lee, Won Ho; Ko, Young-Gyu; Eunice, Eunkyeong Kim; Hwang, Kwang Yeon.
In: Protein and Peptide Letters, Vol. 13, No. 9, 19.09.2006, p. 945-949.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression, purification, and preliminary X-ray crystallographic analysis of the complex of Gαi3-RGS5 from human with GDP/Mg 2+/AlF4 -
AU - Rhee, Kyung Hee
AU - Nam, Ki Hyun
AU - Lee, Won Ho
AU - Ko, Young-Gyu
AU - Eunice, Eunkyeong Kim
AU - Hwang, Kwang Yeon
PY - 2006/9/19
Y1 - 2006/9/19
N2 - Regulator of G-protein signaling 5 (RGS5), an inhibitor of Gq and Gi activation, is a member of the small RGS protein subfamily. However, despite significant process in the investigation of RGS5, no structure is yet available. In order to elucidate the mechanism of the RGS5 in G protein signaling pathway, we have overexpressed the RGS5 and G_i3 from human in Escherichia coli and crystallized the complex of RGS5 and Gαi3 proteins with GDP/Mg2+/AlF4 - at 3.0 Å resolution using a synchrotron radiation source. The complex crystals belong to the tetragonal space group P41212 or P432 12, with unit cell parameters a=b=95.9 Å, and c=138.8 Å. Assuming one complex protein in the crystallographic asymmetric unit, the calculated Matthews parameter (VM) is 2.57 Å3/Da and solvent content is 52.2%.
AB - Regulator of G-protein signaling 5 (RGS5), an inhibitor of Gq and Gi activation, is a member of the small RGS protein subfamily. However, despite significant process in the investigation of RGS5, no structure is yet available. In order to elucidate the mechanism of the RGS5 in G protein signaling pathway, we have overexpressed the RGS5 and G_i3 from human in Escherichia coli and crystallized the complex of RGS5 and Gαi3 proteins with GDP/Mg2+/AlF4 - at 3.0 Å resolution using a synchrotron radiation source. The complex crystals belong to the tetragonal space group P41212 or P432 12, with unit cell parameters a=b=95.9 Å, and c=138.8 Å. Assuming one complex protein in the crystallographic asymmetric unit, the calculated Matthews parameter (VM) is 2.57 Å3/Da and solvent content is 52.2%.
KW - Crystallization
KW - G protein signaling pathway
KW - Gαi
KW - Regulator of G-protein signaling 5
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UR - http://www.scopus.com/inward/citedby.url?scp=33748602906&partnerID=8YFLogxK
U2 - 10.2174/092986606778256225
DO - 10.2174/092986606778256225
M3 - Article
C2 - 17100651
AN - SCOPUS:33748602906
VL - 13
SP - 945
EP - 949
JO - Protein and Peptide Letters
JF - Protein and Peptide Letters
SN - 0929-8665
IS - 9
ER -