Expression, purification, and preliminary X-ray crystallographic analysis of the complex of Gαi3-RGS5 from human with GDP/Mg 2+/AlF4 -

Kyung Hee Rhee, Ki Hyun Nam, Won Ho Lee, Young-Gyu Ko, Eunkyeong Kim Eunice, Kwang Yeon Hwang

Research output: Contribution to journalArticle

Abstract

Regulator of G-protein signaling 5 (RGS5), an inhibitor of Gq and Gi activation, is a member of the small RGS protein subfamily. However, despite significant process in the investigation of RGS5, no structure is yet available. In order to elucidate the mechanism of the RGS5 in G protein signaling pathway, we have overexpressed the RGS5 and G_i3 from human in Escherichia coli and crystallized the complex of RGS5 and Gαi3 proteins with GDP/Mg2+/AlF4 - at 3.0 Å resolution using a synchrotron radiation source. The complex crystals belong to the tetragonal space group P41212 or P432 12, with unit cell parameters a=b=95.9 Å, and c=138.8 Å. Assuming one complex protein in the crystallographic asymmetric unit, the calculated Matthews parameter (VM) is 2.57 Å3/Da and solvent content is 52.2%.

Original languageEnglish
Pages (from-to)945-949
Number of pages5
JournalProtein and Peptide Letters
Volume13
Issue number9
DOIs
Publication statusPublished - 2006 Sep 19

Fingerprint

GTP-Binding Protein Regulators
Purification
X-Rays
X rays
RGS Proteins
Synchrotrons
Synchrotron radiation
GTP-Binding Proteins
Escherichia coli
Proteins
Chemical activation
tetrafluoroaluminate
human RGS5 protein
Radiation
Crystals

Keywords

  • Crystallization
  • G protein signaling pathway
  • Gαi
  • Regulator of G-protein signaling 5

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Expression, purification, and preliminary X-ray crystallographic analysis of the complex of Gαi3-RGS5 from human with GDP/Mg 2+/AlF4 - . / Rhee, Kyung Hee; Nam, Ki Hyun; Lee, Won Ho; Ko, Young-Gyu; Eunice, Eunkyeong Kim; Hwang, Kwang Yeon.

In: Protein and Peptide Letters, Vol. 13, No. 9, 19.09.2006, p. 945-949.

Research output: Contribution to journalArticle

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