Expression, Purification, Characterization and Crystallization of Flap Endonuclease-1 from Methanococcus jannaschii

KeunWook Bae, KyuWon Baek, Chun Seok Cho, Kwang Yeon Hwang, Hak Ryul Kim, Ha Chin Sung, Yunje Cho

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A gene coding for a protein homologous to the flap endonuclease-1 (FEN-1) was cloned from Methanococcus jannaschii, overexpressed, purified and characterized. The gene product from M. jannaschii shows 5′ endo-/exonuclease and 5′ pseudo-Y-endonuclease activities as observed in the FEN-1 in eukaryotes. In addition, Methanococcus jannaschii FEN-1 functions effectively at high concentrations of salt, unlike eukaryotic FEN-1. We have crystallized Methanococcus jannaschii FEN-1 and analyzed its preliminary character. The crystal belongs to the space group of P2 1 with unit cell dimensions of a = 58.93 Å, b = 42.53 Å, c = 62.62 Å and β = 92.25°. A complete data set has been collected at 2.0 Å resolution using a frozen crystal.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalMolecules and Cells
Volume9
Issue number1
Publication statusPublished - 1999 Feb 28
Externally publishedYes

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Keywords

  • Crystallization
  • Flap Endonuclease-1
  • Methanococcus janaschii
  • Structure Specific Nuclease

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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