Expressional diversity of wheat nsLTP genes: Evidence of subfunctionalization via cis-regulatory divergence

Hong Wei Wang, Hyuk Jin Kwon, Won Cheol Yim, Sung Don Lim, Jun Cheol Moon, Byung Moo Lee, Yong Weon Seo, Wook Kim, Cheol Seong Jang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Previously, the wheat non-specific lipid transfer proteins (TaLTP), members of a small multigene family, were reported to evidence a complex pattern of expression regulation. In order to assess further the expression diversity of the TaLTP genes, we have attempted to evaluate their expression profiles in responses to abiotic stresses, using semi-quantitative RT-PCR. The expression profiles generated herein revealed that the TaLTP genes in group A evidenced highly similar responses against abiotic stresses, whereas differential expression patterns among genes in each group were also observed. A total of seven promoters were fused to a GUS reporter gene and the recombinants were introduced into Arabidopsis, while three promoters evidenced non-detectible GUS activity. The promoters of TaLTP1, TaLTP7, and TaLTP10 included in group A drove strong expressions during plant development with overlapping patterns, in large part, but also exhibited distinct expression pattern, thereby suggesting subfunctionalization processing over evolutionary time. However, only trace expression in cotyledons, young emerged leaves, and epidermal cell layers of flower ovaries was driven by the promoter of TaLTP3 of group B. These results indicate that their distinct physiological functions appear to be accomplished by a subfunctionalization process involving degenerative mutations in regulatory regions.

Original languageEnglish
Pages (from-to)843-852
Number of pages10
JournalGenetica
Volume138
Issue number8
DOIs
Publication statusPublished - 2010

Keywords

  • Evolution
  • Gene family
  • Non-specific lipid transfer protein
  • Subfunctionalization
  • cis-element

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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  • Cite this

    Wang, H. W., Kwon, H. J., Yim, W. C., Lim, S. D., Moon, J. C., Lee, B. M., Seo, Y. W., Kim, W., & Jang, C. S. (2010). Expressional diversity of wheat nsLTP genes: Evidence of subfunctionalization via cis-regulatory divergence. Genetica, 138(8), 843-852. https://doi.org/10.1007/s10709-010-9467-7