Abstract
Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 μm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 μm. Tomograms with improved focal depth are demonstrated for single HT29 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 171-173 |
| Number of pages | 3 |
| Journal | Optics Letters |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 Jan 15 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Extended depth of focus in tomographic phase microscopy using a propagation algorithm. / Choi, Wonshik; Fang-Yen, Christopher; Badizadegan, Kamran; Dasari, Ramachandra R.; Feld, Michael S.
In: Optics Letters, Vol. 33, No. 2, 15.01.2008, p. 171-173.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extended depth of focus in tomographic phase microscopy using a propagation algorithm
AU - Choi, Wonshik
AU - Fang-Yen, Christopher
AU - Badizadegan, Kamran
AU - Dasari, Ramachandra R.
AU - Feld, Michael S.
PY - 2008/1/15
Y1 - 2008/1/15
N2 - Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 μm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 μm. Tomograms with improved focal depth are demonstrated for single HT29 cells.
AB - Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 μm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 μm. Tomograms with improved focal depth are demonstrated for single HT29 cells.
UR - http://www.scopus.com/inward/record.url?scp=40149086347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40149086347&partnerID=8YFLogxK
U2 - 10.1364/OL.33.000171
DO - 10.1364/OL.33.000171
M3 - Article
VL - 33
SP - 171
EP - 173
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 2
ER -