Extended depth of focus in tomographic phase microscopy using a propagation algorithm

Wonshik Choi; Christopher Fang-Yen; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1364/OL.33.000171

Extended depth of focus in tomographic phase microscopy using a propagation algorithm

Wonshik Choi, Christopher Fang-Yen, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 μm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 μm. Tomograms with improved focal depth are demonstrated for single HT29 cells.

Original language	English
Pages (from-to)	171-173
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	2
DOIs	https://doi.org/10.1364/OL.33.000171
Publication status	Published - 2008 Jan 15

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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AU - Dasari, Ramachandra R.

AU - Feld, Michael S.

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AB - Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 μm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 μm. Tomograms with improved focal depth are demonstrated for single HT29 cells.

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Extended depth of focus in tomographic phase microscopy using a propagation algorithm

Abstract

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