Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery

Yoosoo Yang, Yeonsun Hong, Eunji Cho, Gi Beom Kim, In San Kim

Research output: Contribution to journalReview article

37 Citations (Scopus)

Abstract

Membrane proteins are of great research interest, particularly because they are rich in targets for therapeutic application. The suitability of various membrane proteins as targets for therapeutic formulations, such as drugs or antibodies, has been studied in preclinical and clinical studies. For therapeutic application, however, a protein must be expressed and purified in as close to its native conformation as possible. This has proven difficult for membrane proteins, as their native conformation requires the association with an appropriate cellular membrane. One solution to this problem is to use extracellular vesicles as a display platform. Exosomes and microvesicles are membranous extracellular vesicles that are released from most cells. Their membranes may provide a favourable microenvironment for membrane proteins to take on their proper conformation, activity, and membrane distribution; moreover, membrane proteins can cluster into microdomains on the surface of extracellular vesicles following their biogenesis. In this review, we survey the state-of-the-art of extracellular vesicle (exosome and small-sized microvesicle)-based therapeutics, evaluate the current biological understanding of these formulations, and forecast the technical advances that will be needed to continue driving the development of membrane protein therapeutics.

Original languageEnglish
Article number1440131
JournalJournal of Extracellular Vesicles
Volume7
Issue number1
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • Exosomes
  • Extracellular vesicle
  • Membrane protein
  • Microdomain
  • Protein therapeutics

ASJC Scopus subject areas

  • Histology
  • Cell Biology

Fingerprint Dive into the research topics of 'Extracellular vesicles as a platform for membrane-associated therapeutic protein delivery'. Together they form a unique fingerprint.

  • Cite this