Extraction and chromatographic separation of anticarcinogenic fractions from cacao bean husk

Won Lee Ki, Eun S. Hwang, Joo Kang Nam, Kyoung Heon Kim, Joo Lee Hyong

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The utilization of cacao bean husk (CBH), a by-product of chocolate manufacture, would be both environmentally and economically beneficial. For this purpose, a process for effectively separating and fractionating CBH fractions having cancer preventive potential was developed in this study. For screening the fractions with potent cancer preventive activity, we examined the effect of extracts and fractions of CBH on the inhibition of gap-junction intercellular communication (GJIC) and the DNA synthesis of cancer cells, both of which are characteristics of the promotion and progression stages in carcinogenesis. The extracts of CBH (especially, the 60% ethanol fraction after extraction with 50% acetone) containing 43 wt.% polyphenol exerted an excellent protective effect on H2O2-induced inhibition of GJIC in WB-F344 rat liver epithelial cells as determined by the scrape-loading/dye transfer assay. The enhancement of GJIC by the extracts of CBH was approximately 10-fold higher than that of a well-known dietary chemopreventive component, vitamin C. The extracts of CBH (especially, the 60% ethanol fraction) also suppressed DNA synthesis in all liver, stomach, and colon cancer cells as demonstrated by the 3H-thymidine incorporation assay, by approximately four-fold higher than that of vitamin C. These results imply that the polyphenol extracts and fractions of CBH are effective functional materials to be used in either preventing or inhibiting cancer.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalBioFactors
Volume23
Issue number3
DOIs
Publication statusPublished - 2005

Fingerprint

Cacao
Polyphenols
Liver
Ascorbic Acid
Assays
Communication
Ethanol
Cells
Gap Junctions
Functional materials
DNA
Acetone
Thymidine
Byproducts
Rats
Screening
Coloring Agents
Neoplasms
Inbred F344 Rats
Liver Neoplasms

Keywords

  • Cacao bean husk
  • Cancer chemoprevention
  • Cell proliferation
  • Gap-junction intercellular communication
  • Polyphenol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Extraction and chromatographic separation of anticarcinogenic fractions from cacao bean husk. / Ki, Won Lee; Hwang, Eun S.; Nam, Joo Kang; Kim, Kyoung Heon; Hyong, Joo Lee.

In: BioFactors, Vol. 23, No. 3, 2005, p. 141-150.

Research output: Contribution to journalArticle

Ki, Won Lee ; Hwang, Eun S. ; Nam, Joo Kang ; Kim, Kyoung Heon ; Hyong, Joo Lee. / Extraction and chromatographic separation of anticarcinogenic fractions from cacao bean husk. In: BioFactors. 2005 ; Vol. 23, No. 3. pp. 141-150.
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