Extraction and fractionation of glucosyltransferase inhibitors from cacao bean husk

Kyoung Heon Kim, Ki Won Lee, Dong Young Kim, Hyung Hwan Park, Ik Boo Kwon, Hyong Joo Lee

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


The re-utilization of cacao bean husk, a waste generated from the chocolate industry, would bring benefits both environmentaly and economicaly. This study relates to a process for effectively separating and fractionating a cacao bean husk fraction having high inhibitory activity against glucosyltransferase (GTF) for the prevention of tooth decay (anticaries activity). Since the GTF inhibitory activity is known to be rendered by polyphenols, the separation process was also able to aim at high recovery of polyphenols which benefits human health. In this study, cacao bean husk extract, obtained under optimal conditions, extraction with 50% (v/v) aq. (aqueous) acetone solution at 60°C for 4 h followed by 50% (v/v) aq. ethanol using a styrene-based resin, showed significantly higher inhibitory activity (2 and 12 folds, respectively) against GTF and a similar polyphenol content, compared to two other commercial anti-GTF polyphenol products.

Original languageEnglish
Pages (from-to)2043-2046
Number of pages4
JournalProcess Biochemistry
Issue number12
Publication statusPublished - 2004 Oct 29


  • Anticaries
  • Cacao bean husk
  • Glucosyltransferase
  • Polyphenols
  • Theobroma cacao

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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