Extraction of three-dimensional information of biological membranous tissue with scanning confocal infrared laser microscope tomography

Soonwook Kwon, Se Bum Choi, Min Gyu Park, Hyunung Yu, Seung Woo Suh, Im Joo Rhyu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The LEXT confocal laser scanning microscope has been used for the three-dimensional (3D) imaging of the surface of specimens, especially in materials science fields, by the penetration of near-infrared (NIR) light without mechanical cutting, deposition, or other specimen pretreatment. Noninvasive investigation of various biological tissues such as human spinal dura mater, rat aorta, and cornea without the dehydration process was successfully carried out with the LEXT, in order to access both surface and internal topographic images of the biological structures at a good status of the wet tissue such as in vivo, especially in measuring tissue thickness. The confocal NIR laser microscopy offers the viable means to visualize tissue architecture and its thickness in microdomain to integrate 3D images efficiently. We believe that the LEXT has a good application for biological researchers to study biomaterials, and it would be useful as a diagnostic tool in the near future.

Original languageEnglish
Pages (from-to)194-197
Number of pages4
JournalMicroscopy and Microanalysis
Volume19
Issue numberSUPPL. 5
DOIs
Publication statusPublished - 2013 Aug

Keywords

  • confocal
  • dura mater
  • imaging
  • infrared
  • laser microscope
  • membrane
  • scanning

ASJC Scopus subject areas

  • Instrumentation

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