Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036

Sun Young Choi, Gyeongtaek Gong, Hong Sil Park, Youngsoon Um, Sang Jun Sim, Han Min Woo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. Here we isolated an extreme furfural-tolerant bacterium Enterobacter cloacae GGT036 from soil sample collected in Mt. Gwanak, Republic of Korea. Among isolated bacteria, only E. cloacae GGT036 showed cell growth with 35mM furfural under aerobic culture. Compared to the maximal half inhibitory concentration (IC50) of well-known industrial strains Escherichia coli (24.9mM furfural) and Corynebacterium glutamicum (10mM furfural) based on the cell density, IC50 of E. cloacae GGT036 (47.7mM) was significantly higher after 24h, compared to E. coli and C. glutamicum. Since bacterial cell growth was exponentially inhibited depending on linearly increased furfural concentrations in the medium, we concluded that E. cloacae GGT036 is an extreme furfural-tolerant bacterium. Recently, the complete genome sequence of E. cloacae GGT036 was announced and this could provide an insight for engineering of E. cloacae GGT036 itself or other industrially relevant bacteria.

Original languageEnglish
Pages (from-to)11-13
Number of pages3
JournalJournal of Biotechnology
Volume193
DOIs
Publication statusPublished - 2015 Jan 1

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Furaldehyde
Enterobacter cloacae
Soil
Bacteria
Corynebacterium glutamicum
Inhibitory Concentration 50
Escherichia coli
Republic of Korea
Growth
Biomass
Cell Count
Genome

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036. / Choi, Sun Young; Gong, Gyeongtaek; Park, Hong Sil; Um, Youngsoon; Sim, Sang Jun; Woo, Han Min.

In: Journal of Biotechnology, Vol. 193, 01.01.2015, p. 11-13.

Research output: Contribution to journalArticle

Choi, Sun Young ; Gong, Gyeongtaek ; Park, Hong Sil ; Um, Youngsoon ; Sim, Sang Jun ; Woo, Han Min. / Extreme furfural tolerance of a soil bacterium Enterobacter cloacae GGT036. In: Journal of Biotechnology. 2015 ; Vol. 193. pp. 11-13.
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