Extremely low-frequency electromagnetic field induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3

Anastasia Rosebud Aikins, Sung Won Hong, Hyun Jung Kim, Cheol Ho Yoon, Joo Hee Chung, Mijung Kim, Chan Wha Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Extremely low-frequency electromagnetic field (ELFEMF) can stimulate neural differentiation in human bone marrow-derived mesenchymal cells (hBM-MSCs), and this provides an opportunity for research on neurodegenerative diseases such as Alzheimer's disease (AD). Metallothionein-3 (MT3), an isoform of the metal-binding proteins, metallothioneins, involved in maintaining intracellular zinc (Zn) homeostasis and the deregulation of zinc homeostasis, has separately been implicated in AD. Here, we investigated the effect of ELFEMF-induced neural differentiation of hBM-MSCs on Zn-MT3 homeostatic interaction. Exposure to ELFEMF induced neural differentiation of hBM-MSCs, which was characterized by decreased proliferation and enhanced neural-like morphology. We observed expression of neuronal markers such as β-tubulin3, pleiotrophin, and neurofilament-M at the mRNA level and MAP2 at the protein level. ELFEMF-induced neural differentiation correlated with decreased expression of metal-response element-transcription factor 1 and MT3, as well as decreased intracellular Zn concentration. In addition, upregulation of dihydropyrimidinase-related protein 2 was observed, but there was no change in γ-enolase expression. These data indicate a possible regulatory mechanism for MT3 during neural differentiation. Our findings provide considerable insight into molecular mechanisms involved in neural differentiation, which is useful for developing new treatments for neurodegenerative diseases. Bioelectromagnetics.

Original languageEnglish
JournalBioelectromagnetics
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Electromagnetic Fields
Bone Marrow
Zinc
Neurodegenerative Diseases
Alzheimer Disease
Homeostasis
Metals
Intermediate Filaments
Phosphopyruvate Hydratase
Metallothionein
Response Elements
Carrier Proteins
Protein Isoforms
Transcription Factors
Up-Regulation
Messenger RNA
growth inhibitory factor
zinc thionein
Research
Proteins

Keywords

  • Alzheimer's disease
  • HPLC-ICP-MS
  • Intracellular zinc homeostasis
  • Metal-binding proteins
  • Neural markers

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Radiology Nuclear Medicine and imaging

Cite this

Extremely low-frequency electromagnetic field induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3. / Aikins, Anastasia Rosebud; Hong, Sung Won; Kim, Hyun Jung; Yoon, Cheol Ho; Chung, Joo Hee; Kim, Mijung; Kim, Chan Wha.

In: Bioelectromagnetics, 2017.

Research output: Contribution to journalArticle

Aikins, Anastasia Rosebud ; Hong, Sung Won ; Kim, Hyun Jung ; Yoon, Cheol Ho ; Chung, Joo Hee ; Kim, Mijung ; Kim, Chan Wha. / Extremely low-frequency electromagnetic field induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3. In: Bioelectromagnetics. 2017.
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