Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells

Hyun Jung Kim, Jessica Jung, Jee Hye Park, Jin Hee Kim, Kyung Nam Ko, Chan Wha Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Extremely low-frequency electromagnetic fields (ELF-EMF) affect numerous biological functions such as gene expression, cell fate determination and even cell differentiation. To investigate the correlation between ELF-EMF exposure and differentiation, bone marrow derived mesenchymal stem cells (BM-MSCs) were subjected to a 50-Hz electromagnetic field during in vitro expansion. The influence of ELF-EMF on BM-MSCs was analysed by a range of different analytical methods to understand its role in the enhancement of neural differentiation. ELF-EMF exposure significantly decreased the rate of proliferation, which in turn caused an increase in neuronal differentiation. The ELF-EMF-treated cells showed increased levels of neuronal differentiation marker (MAP2), while early neuronal marker (Nestin) was down-regulated. In addition, eight differentially expressed proteins were detected in two-dimensional electrophoresis maps, and were identified using ESI-Q-TOF LC/MS/MS. Among them, ferritin light chain, thioredoxin-dependent peroxide reductase, and tubulin β-6 chain were up-regulated in the ELF-EMF-stimulated group. Ferritin and thioredoxin-dependent peroxide reductase are involved in a wide variety of functions, including Ca2+ regulation, which is a critical component of neurodegeneration. We also observed that the intracellular Ca2+ content was significantly elevated after ELF-EMF exposure, which strengthens the modulatory role of ferritin and thioredoxin-dependent peroxide reductase, during differentiation. Notably, western blot analysis indicated significantly increased expression of the ferritin light chain in the ELF-EMF-stimulated group (0.60 vs. 1.08; P < 0.01). These proteins may help understand the effect of ELF-EMF stimulation on BM-MSCs during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases.

Original languageEnglish
Pages (from-to)923-931
Number of pages9
JournalExperimental Biology and Medicine
Volume238
Issue number8
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Electromagnetic Fields
Stem cells
Mesenchymal Stromal Cells
Electromagnetic fields
Bone
Bone Marrow
Thioredoxins
Peroxides
Apoferritins
Oxidoreductases
Ferritins
Neurodegenerative diseases
Nestin
Differentiation Antigens
Tubulin
Electrophoresis
Gene expression
Neurodegenerative Diseases
Cell Differentiation
Proteins

Keywords

  • BM-MSCs
  • Ca2+ regulation
  • Extremely low-frequency electromagnetic fields
  • ferritin
  • neural differentiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells. / Kim, Hyun Jung; Jung, Jessica; Park, Jee Hye; Kim, Jin Hee; Ko, Kyung Nam; Kim, Chan Wha.

In: Experimental Biology and Medicine, Vol. 238, No. 8, 01.08.2013, p. 923-931.

Research output: Contribution to journalArticle

Kim, Hyun Jung ; Jung, Jessica ; Park, Jee Hye ; Kim, Jin Hee ; Ko, Kyung Nam ; Kim, Chan Wha. / Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells. In: Experimental Biology and Medicine. 2013 ; Vol. 238, No. 8. pp. 923-931.
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