Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells

Mi Na Hong, Na Kyung Han, Hyung Chul Lee, Young Kyu Ko, Sung Gil Chi, Yun Sil Lee, Yoon Myung Gimm, Sung Ho Myung, Jae Seon Lee

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalJournal of Radiation Research
Volume53
Issue number1
DOIs
Publication statusPublished - 2012

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

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