Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells

Mi Na Hong, Na Kyung Han, Hyung Chul Lee, Young-Gyu Ko, Sung-Gil Chi, Yun Sil Lee, Yoon Myung Gimm, Sung Ho Myung, Jae Seon Lee

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17 Citations (Scopus)

Abstract

The aim of this study was to determine whether extremely low frequency magnetic fields (ELF-MF) could affect intracellular reactive oxygen species (ROS) levels and antioxidant enzyme activity. After MCF10A human breast epithelial cells were exposed to 1 mT of 60 Hz ELF-MF for 4 hours, intracellular ROS level, superoxide dismutase (SOD) activity, and reduced to oxidized glutathione (GSH/GSSG) ratio were measured. The cells exposed to ELF-MF did not evidence statistically significant changes in the above-mentioned biological parameters as compared to either the incubator controls or sham-exposed cells. By way of contrast, the IR-exposed cells exhibited marked changes in ROS level, SOD activity, and GSH/GSSG ratio. When we assessed morphological changes and senescence-associated beta-galactosidase (SA-β-Gal) activity, only the IR-exposed cells were positive. According to our results, it could be concluded that ELF-MF has no effect on intracellular ROS level, SOD activity, and GSH/GSSG ratio under our exposure condition.

Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalJournal of Radiation Research
Volume53
Issue number1
DOIs
Publication statusPublished - 2012 Mar 12

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiation
  • Health, Toxicology and Mutagenesis

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    Hong, M. N., Han, N. K., Lee, H. C., Ko, Y-G., Chi, S-G., Lee, Y. S., Gimm, Y. M., Myung, S. H., & Lee, J. S. (2012). Extremely low frequency magnetic fields do not elicit oxidativestress in MCF10A cells. Journal of Radiation Research, 53(1), 79-86. https://doi.org/10.1269/jrr.11049