Fabrication and characterization of silicon-based photodiode for detection of luminol chemiluminescence in a biosensor

Hyung Il Kim, Chul Goo Kang, Moon Sik Kang, Jung Ho Park, Nam Ki Min

Research output: Contribution to journalConference article

4 Citations (Scopus)

Abstract

In this paper, a silicon-based p+-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H2O2) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H2O2 concentration under 2 mM luminol and 25 units HRP. As the H2O2 concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H2O2 concentration in the range 0.01 ∼ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM.mm2.

Original languageEnglish
Pages (from-to)S336-S339
JournalJournal of the Korean Physical Society
Volume42
Issue numberSPEC.
Publication statusPublished - 2003 Feb 1
EventProceedings of The 11th Seoul International Symposium on the Physics of Semiconductors and Apllications - 2002 - Cheju Island, Korea, Republic of
Duration: 2002 Aug 202002 Aug 23

Keywords

  • Biosensor
  • Chemiluminescence
  • Luminol
  • Photodiode

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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