In this paper, a silicon-based p+-n junction photodiode is fabricated by semiconductor processing as a detector for luminol chemiluminescence (CL) that is used in present the biosensor. The optimum conditions of the CL reaction are determined by horse-radish peroxidase (HRP) concentration, the reagent mixture ratio of luminol with HRP and hydrogen peroxide (H2O2) in the reactor well made of polydimethylsiloxane (PDMS). The output current of the photodiode is measured for CL by H2O2 concentration under 2 mM luminol and 25 units HRP. As the H2O2 concentration increases, the photodiode output current peak values increase. The photocurrent is linearly related to the H2O2 concentration in the range 0.01 ∼ 0.5 mM, and then the sensitivity of the photodiode is 23.429 pA/mM.mm2.
|Journal||Journal of the Korean Physical Society|
|Publication status||Published - 2003 Feb|
|Event||Proceedings of The 11th Seoul International Symposium on the Physics of Semiconductors and Apllications - 2002 - Cheju Island, Korea, Republic of|
Duration: 2002 Aug 20 → 2002 Aug 23
ASJC Scopus subject areas
- Physics and Astronomy(all)